CDC19/YAL038W Protein Information

Standard Name	Cdc19p 1
Systematic Name	Yal038wp
Alias	Pyk1p 2
ORF Classification	Verified
Description	Pyruvate kinase; functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvate, the input for aerobic (TCA cycle) or anaerobic (glucose fermentation) respiration; regulated via allosteric activation by fructose bisphosphate; CDC19 has a paralog, PYK2, that arose from the whole genome duplication (3, 4, 5)
Name Description	Cell Division Cycle 6

Experimental Data
Molecules/cell	291000 7

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	500
Molecular Weight (Da)	54,544
Isoelectric Point (pI)	7.66

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Cdc19p (InterPro)
Physical Interactions	There are 123 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000011038E \| P00549 MIPS: YAL038W NCBI: 125609 \| 3212328 \| 3212329 \| 3212330 \| 3212331 \| 4180 \| 51013665 \| 5265 \| 595546 \| 63028795 \| 63028797 \| 63028799 \| 63028801 \| 63028803 \| 63028805 \| 63028807 \| 63028809 \| 63028811 \| 63028813 \| 63028815 \| 63028817 \| 63028819 \| 63028821 \| 63028823 \| 63028825 \| 63028827 \| 63028829 \| 63028831 \| 63028833 \| 63028835 \| 63028837 \| 63028839 \| 63028841 \| 63028843 \| 63028845 \| 63028847 \| 63028849 \| 63028851 \| 6319279 \| NP_009362.1 \| NM_001178183.1 GenBank/EMBL/DDBJ: DAA06948.1 \| AY693107 \| AY949862 \| AY949863 \| AY949864 \| AY949865 \| AY949866 \| AY949867 \| AY949868 \| AY949869 \| AY949870 \| AY949871 \| AY949872 \| AY949873 \| AY949874 \| AY949875 \| AY949876 \| AY949877 \| AY949878 \| AY949879 \| AY949880 \| AY949881 \| AY949882 \| AY949883 \| AY949884 \| AY949885 \| AY949886 \| AY949887 \| AY949888 \| AY949889 \| AY949890 \| U12980 \| V01321 \| X14400
External Classifications	EC: 2.7.1.40 [Pyruvate kinase]

Amino Acid Sequence (or in FASTA format)
1 MSRLERLTSL NVVAGSDLRR TSIIGTIGPK TNNPETLVAL RKAGLNIVRM 51 NFSHGSYEYH KSVIDNARKS EELYPGRPLA IALDTKGPEI RTGTTTNDVD 101 YPIPPNHEMI FTTDDKYAKA CDDKIMYVDY KNITKVISAG RIIYVDDGVL 151 SFQVLEVVDD KTLKVKALNA GKICSHKGVN LPGTDVDLPA LSEKDKEDLR 201 FGVKNGVHMV FASFIRTAND VLTIREVLGE QGKDVKIIVK IENQQGVNNF 251 DEILKVTDGV MVARGDLGIE IPAPEVLAVQ KKLIAKSNLA GKPVICATQM 301 LESMTYNPRP TRAEVSDVGN AILDGADCVM LSGETAKGNY PINAVTTMAE 351 TAVIAEQAIA YLPNYDDMRN CTPKPTSTTE TVAASAVAAV FEQKAKAIIV 401 LSTSGTTPRL VSKYRPNCPI ILVTRCPRAA RFSHLYRGVF PFVFEKEPVS 451 DWTDDVEARI NFGIEKAKEF GILKKGDTYV SIQGFKAGAG HSNTLQVSTV 501 *

Amino Acid Sequence (or in FASTA format)

       1  MSRLERLTSL NVVAGSDLRR TSIIGTIGPK TNNPETLVAL RKAGLNIVRM
      51  NFSHGSYEYH KSVIDNARKS EELYPGRPLA IALDTKGPEI RTGTTTNDVD
     101  YPIPPNHEMI FTTDDKYAKA CDDKIMYVDY KNITKVISAG RIIYVDDGVL
     151  SFQVLEVVDD KTLKVKALNA GKICSHKGVN LPGTDVDLPA LSEKDKEDLR
     201  FGVKNGVHMV FASFIRTAND VLTIREVLGE QGKDVKIIVK IENQQGVNNF
     251  DEILKVTDGV MVARGDLGIE IPAPEVLAVQ KKLIAKSNLA GKPVICATQM
     301  LESMTYNPRP TRAEVSDVGN AILDGADCVM LSGETAKGNY PINAVTTMAE
     351  TAVIAEQAIA YLPNYDDMRN CTPKPTSTTE TVAASAVAAV FEQKAKAIIV
     401  LSTSGTTPRL VSKYRPNCPI ILVTRCPRAA RFSHLYRGVF PFVFEKEPVS
     451  DWTDDVEARI NFGIEKAKEF GILKKGDTYV SIQGFKAGAG HSNTLQVSTV
     501  *

external links for Cdc19p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastGFP
Candida (CGD)	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
Candida (CandidaDB)	Complexome	YeastRC Structure Prediction (Seattle)
YGOB	DIP
YOGY	GeneMANIA
	YeastRC Mass Spec (Seattle)

References cited on this page View Complete Literature Guide for Cdc19p

1)	Link, A. and Olson, M. (1989) Personal Communication, Mortimer Map Edition 10
2)	Sprague GF Jr (1977) Isolation and characterization of a Saccharomyces cerevisiae mutant deficient in pyruvate kinase activity. J Bacteriol 130(1):232-41
3)	Pearce AK, et al. (2001) Pyruvate kinase (Pyk1) levels influence both the rate and direction of carbon flux in yeast under fermentative conditions. Microbiology 147(Pt 2):391-401
4)	Byrne KP and Wolfe KH (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
5)	Xu YF, et al. (2012) Regulation of yeast pyruvate kinase by ultrasensitive allostery independent of phosphorylation. Mol Cell 48(1):52-62
6)	Hartwell LH, et al. (1970) Genetic control of the cell-division cycle in yeast. I. Detection of mutants. Proc Natl Acad Sci U S A 66(2):352-9
7)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41