RBG1/YAL036C Protein Information

Standard Name	Rbg1p 1, 2
Systematic Name	Yal036cp
Alias	Fun11p 3
ORF Classification	Verified
Description	Member of the DRG family of GTP-binding proteins; associates with translating ribosomes; interacts with Tma46p, Ygr250cp, Gir2p and Yap1p via two-hybrid (2, 4, 5, 6)
Name Description	RiBosome interacting Gtpase 1

Experimental Data
Molecules/cell	11700 7

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	369
Molecular Weight (Da)	40,701
Isoelectric Point (pI)	8.49

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Rbg1p (InterPro)
Physical Interactions	There are 38 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000005318D \| P39729 MIPS: YAL036C NCBI: 407943709 \| 407943710 \| 51013535 \| 595548 \| 6319281 \| 731276 \| NP_009364.1 GenBank/EMBL/DDBJ: DAA06951.1 \| AY693042 \| U12980

Amino Acid Sequence (or in FASTA format)
1 MSTTVEKIKA IEDEMARTQK NKATSFHLGQ LKAKLAKLRR ELLTSASSGS 51 GGGAGIGFDV ARTGVASVGF VGFPSVGKST LLSKLTGTES EAAEYEFTTL 101 VTVPGVIRYK GAKIQMLDLP GIIDGAKDGR GRGKQVIAVA RTCNLLFIIL 151 DVNKPLHHKQ IIEKELEGVG IRLNKTPPDI LIKKKEKGGI SITNTVPLTH 201 LGNDEIRAVM SEYRINSAEI AFRCDATVDD LIDVLEASSR RYMPAIYVLN 251 KIDSLSIEEL ELLYRIPNAV PISSGQDWNL DELLQVMWDR LNLVRIYTKP 301 KGQIPDFTDP VVLRSDRCSV KDFCNQIHKS LVDDFRNALV YGSSVKHQPQ 351 YVGLSHILED EDVVTILKK*

Amino Acid Sequence (or in FASTA format)

       1  MSTTVEKIKA IEDEMARTQK NKATSFHLGQ LKAKLAKLRR ELLTSASSGS
      51  GGGAGIGFDV ARTGVASVGF VGFPSVGKST LLSKLTGTES EAAEYEFTTL
     101  VTVPGVIRYK GAKIQMLDLP GIIDGAKDGR GRGKQVIAVA RTCNLLFIIL
     151  DVNKPLHHKQ IIEKELEGVG IRLNKTPPDI LIKKKEKGGI SITNTVPLTH
     201  LGNDEIRAVM SEYRINSAEI AFRCDATVDD LIDVLEASSR RYMPAIYVLN
     251  KIDSLSIEEL ELLYRIPNAV PISSGQDWNL DELLQVMWDR LNLVRIYTKP
     301  KGQIPDFTDP VVLRSDRCSV KDFCNQIHKS LVDDFRNALV YGSSVKHQPQ
     351  YVGLSHILED EDVVTILKK*

external links for Rbg1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastRC Public Image Repository
Candida (CGD)	CYC2008 (complexes)	Pfam domains
Candida (CandidaDB)	Complexome	YeastRC Structure Prediction (Seattle)
YGOB	DIP
YOGY	GeneMANIA
	YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Rbg1p

1)	Wout, P. and Maddock, J. (2004) The Saccharomyces cerevisiae Rig1p protein and its binding partner Gir2p, interact on polyribosomes with Gcn1p.
2)	Datta K, et al. (2005) The yeast GTPase Mtg2p is required for mitochondrial translation and partially suppresses an rRNA methyltransferase mutant, mrm2. Mol Biol Cell 16(2):954-63
3)	Coleman KG, et al. (1986) Molecular cloning of chromosome I DNA from Saccharomyces cerevisiae: isolation and characterization of the CDC24 gene and adjacent regions of the chromosome. Mol Cell Biol 6(12):4516-25
4)	Li B and Trueb B (2000) DRG represents a family of two closely related GTP-binding proteins. Biochim Biophys Acta 1491(1-3):196-204
5)	Fleischer TC, et al. (2006) Systematic identification and functional screens of uncharacterized proteins associated with eukaryotic ribosomal complexes. Genes Dev 20(10):1294-307
6)	Wout PK, et al. (2009) Saccharomyces cerevisiae Rbg1 protein and its binding partner Gir2 interact on Polyribosomes with Gcn1. Eukaryot Cell 8(7):1061-71
7)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41