FUN12/YAL035W Protein Information

Standard Name	Fun12p 1
Systematic Name	Yal035wp
Alias	Yif2p
ORF Classification	Verified
Description	GTPase required for general translation initiation; promotes Met-tRNAiMet binding to ribosomes and ribosomal subunit joining; promotes GTP-dependent maturation of 18S rRNA by Nob1p; protein abundance increases in response to DNA replication stress; homolog of bacterial IF2 (2, 3, 4, 5)
Name Description	Function Unknown Now 1
Gene Product	eIF5B 6

Experimental Data
Molecules/cell	13400 7

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	1,002
Molecular Weight (Da)	112,268
Isoelectric Point (pI)	5.55

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Fun12p (InterPro)
Physical Interactions	There are 127 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000052E3F \| P39730 MIPS: YAL035W NCBI: 1326056 \| 1723187 \| 460044 \| 6319282 \| NP_009365.1 \| NM_001178180.1 GenBank/EMBL/DDBJ: DAA06952.1 \| L29389 \| U12980

Amino Acid Sequence (or in FASTA format)
1 MAKKSKKNQQ NYWDEEFEED AAQNEEISAT PTPNPESSAG ADDTSREASA 51 SAEGAEAIEG DFMSTLKQSK KKQEKKVIEE KKDGKPILKS KKEKEKEKKE 101 KEKQKKKEQA ARKKAQQQAQ KEKNKELNKQ NVEKAAAEKA AAEKSQKSKG 151 ESDKPSASAK KPAKKVPAGL AALRRQLELK KQLEEQEKLE REEEERLEKE 201 EEERLANEEK MKEEAKAAKK EKEKAKREKR KAEGKLLTRK QKEEKKLLER 251 RRAALLSSGN VKVAGLAKKD GEENKPKKVV YSKKKKRTTQ ENASEAIKSD 301 SKKDSEVVPD DELKESEDVL IDDWENLALG DDDEEGTNEE TQESTASHEN 351 EDQNQGEEEE EGEEEEEEEE ERAHVHEVAK STPAATPAAT PTPSSASPNK 401 KDLRSPICCI LGHVDTGKTK LLDKIRQTNV QGGEAGGITQ QIGATYFPID 451 AIKAKTKVMA EYEKQTFDVP GLLVIDTPGH ESFSNLRSRG SSLCNIAILV 501 IDIMHGLEQQ TIESIKLLRD RKAPFVVALN KIDRLYDWKA IPNNSFRDSF 551 AKQSRAVQEE FQSRYSKIQL ELAEQGLNSE LYFQNKNMSK YVSIVPTSAV 601 TGEGVPDLLW LLLELTQKRM SKQLMYLSHV EATILEVKVV EGFGTTIDVI 651 LSNGYLREGD RIVLCGMNGP IVTNIRALLT PQPLRELRLK SEYVHHKEVK 701 AALGVKIAAN DLEKAVSGSR LLVVGPEDDE DELMDDVMDD LTGLLDSVDT 751 TGKGVVVQAS TLGSLEALLD FLKDMKIPVM SIGLGPVYKR DVMKASTMLE 801 KAPEYAVMLC FDVKVDKEAE QYAEQEGIKI FNADVIYHLF DSFTAYQEKL 851 LEERRKDFLD YAIFPCVLQT LQIINKRGPM IIGVDVLEGT LRVGTPICAV 901 KTDPTTKERQ TLILGKVISL EINHQPVQEV KKGQTAAGVA VRLEDPSGQQ 951 PIWGRHVDEN DTLYSLVSRR SIDTLKDKAF RDQVARSDWL LLKKLKVVFG 1001 IE*

Amino Acid Sequence (or in FASTA format)

       1  MAKKSKKNQQ NYWDEEFEED AAQNEEISAT PTPNPESSAG ADDTSREASA
      51  SAEGAEAIEG DFMSTLKQSK KKQEKKVIEE KKDGKPILKS KKEKEKEKKE
     101  KEKQKKKEQA ARKKAQQQAQ KEKNKELNKQ NVEKAAAEKA AAEKSQKSKG
     151  ESDKPSASAK KPAKKVPAGL AALRRQLELK KQLEEQEKLE REEEERLEKE
     201  EEERLANEEK MKEEAKAAKK EKEKAKREKR KAEGKLLTRK QKEEKKLLER
     251  RRAALLSSGN VKVAGLAKKD GEENKPKKVV YSKKKKRTTQ ENASEAIKSD
     301  SKKDSEVVPD DELKESEDVL IDDWENLALG DDDEEGTNEE TQESTASHEN
     351  EDQNQGEEEE EGEEEEEEEE ERAHVHEVAK STPAATPAAT PTPSSASPNK
     401  KDLRSPICCI LGHVDTGKTK LLDKIRQTNV QGGEAGGITQ QIGATYFPID
     451  AIKAKTKVMA EYEKQTFDVP GLLVIDTPGH ESFSNLRSRG SSLCNIAILV
     501  IDIMHGLEQQ TIESIKLLRD RKAPFVVALN KIDRLYDWKA IPNNSFRDSF
     551  AKQSRAVQEE FQSRYSKIQL ELAEQGLNSE LYFQNKNMSK YVSIVPTSAV
     601  TGEGVPDLLW LLLELTQKRM SKQLMYLSHV EATILEVKVV EGFGTTIDVI
     651  LSNGYLREGD RIVLCGMNGP IVTNIRALLT PQPLRELRLK SEYVHHKEVK
     701  AALGVKIAAN DLEKAVSGSR LLVVGPEDDE DELMDDVMDD LTGLLDSVDT
     751  TGKGVVVQAS TLGSLEALLD FLKDMKIPVM SIGLGPVYKR DVMKASTMLE
     801  KAPEYAVMLC FDVKVDKEAE QYAEQEGIKI FNADVIYHLF DSFTAYQEKL
     851  LEERRKDFLD YAIFPCVLQT LQIINKRGPM IIGVDVLEGT LRVGTPICAV
     901  KTDPTTKERQ TLILGKVISL EINHQPVQEV KKGQTAAGVA VRLEDPSGQQ
     951  PIWGRHVDEN DTLYSLVSRR SIDTLKDKAF RDQVARSDWL LLKKLKVVFG
    1001  IE*

external links for Fun12p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
Candida (CGD)	BioPIXIE	MIPS	YeastGFP
Candida (CandidaDB)	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
YGOB	Complexome	YeastRC Structure Prediction (Seattle)
YOGY	DIP
	GeneMANIA

References cited on this page View Complete Literature Guide for Fun12p

1)	Coleman KG, et al. (1986) Molecular cloning of chromosome I DNA from Saccharomyces cerevisiae: isolation and characterization of the CDC24 gene and adjacent regions of the chromosome. Mol Cell Biol 6(12):4516-25
2)	Choi SK, et al. (1998) Promotion of met-tRNAiMet binding to ribosomes by yIF2, a bacterial IF2 homolog in yeast. Science 280(5370):1757-60
3)	Lee JH, et al. (2002) Initiation factor eIF5B catalyzes second GTP-dependent step in eukaryotic translation initiation. Proc Natl Acad Sci U S A 99(26):16689-94
4)	Lebaron S, et al. (2012) Proofreading of pre-40S ribosome maturation by a translation initiation factor and 60S subunits. Nat Struct Mol Biol 19(8):744-53
5)	Tkach JM, et al. (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
6)	Komar AA, et al. (2005) Novel characteristics of the biological properties of the yeast Saccharomyces cerevisiae eukaryotic initiation factor 2A. J Biol Chem 280(16):15601-11
7)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41