POP5/YAL033W Protein Information

Standard Name	Pop5p 1
Systematic Name	Yal033wp
Alias	Fun53p 2
ORF Classification	Verified
Description	Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs (1, 3, 4)
Name Description	Processing Of Precursor RNAs

Experimental Data
Molecules/cell	2230 5

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	173
Molecular Weight (Da)	19,573
Isoelectric Point (pI)	7.83

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Pop5p (InterPro)
Physical Interactions	There are 37 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000131F39 \| P28005 MIPS: YAL033W NCBI: 120584 \| 5243 \| 595552 \| 6319286 \| NP_009369.1 GenBank/EMBL/DDBJ: DAA06955.1 \| U12980 \| X62577
External Classifications	EC: 3.1.26.5 [Ribonuclease P]

Amino Acid Sequence (or in FASTA format)
1 MVRLKSRYIL FEIIFPPTDT NVEESVSKAD ILLSHHRASP ADVSIKSILQ 51 EIRRSLSLNL GDYGSAKCNS LLQLKYFSNK TSTGIIRCHR EDCDLVIMAL 101 MLMSKIGDVD GLIVNPVKVS GTIKKIEQFA MRRNSKILNI IKCSQSSHLS 151 DNDFIINDFK KIGRENENEN EDD*

Amino Acid Sequence (or in FASTA format)

       1  MVRLKSRYIL FEIIFPPTDT NVEESVSKAD ILLSHHRASP ADVSIKSILQ
      51  EIRRSLSLNL GDYGSAKCNS LLQLKYFSNK TSTGIIRCHR EDCDLVIMAL
     101  MLMSKIGDVD GLIVNPVKVS GTIKKIEQFA MRRNSKILNI IKCSQSSHLS
     151  DNDFIINDFK KIGRENENEN EDD*

external links for Pop5p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastRC Public Image Repository
Candida (CGD)	CYC2008 (complexes)	Pfam domains
Candida (CandidaDB)	Complexome	YeastRC Structure Prediction (Seattle)
YGOB	DIP
YOGY	GeneMANIA
	YeastRC Mass Spec (Seattle)

References cited on this page View Complete Literature Guide for Pop5p

1)	Chamberlain JR, et al. (1998) Purification and characterization of the nuclear RNase P holoenzyme complex reveals extensive subunit overlap with RNase MRP. Genes Dev 12(11):1678-90
2)	Harris SD, et al. (1992) Molecular analysis of Saccharomyces cerevisiae chromosome I. On the number of genes and the identification of essential genes using temperature-sensitive-lethal mutations. J Mol Biol 225(1):53-65
3)	Houser-Scott F, et al. (2002) Interactions among the protein and RNA subunits of Saccharomyces cerevisiae nuclear RNase P. Proc Natl Acad Sci U S A 99(5):2684-9
4)	Marvin MC, et al. (2011) Accumulation of noncoding RNA due to an RNase P defect in Saccharomyces cerevisiae. RNA 17(8):1441-50
5)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41