TPD3/YAL016W Protein Information

Standard Name	Tpd3p
Systematic Name	Yal016wp
Alias	Fun32p
ORF Classification	Verified
Description	Regulatory subunit A of the heterotrimeric protein phosphatase 2A (PP2A), which also contains regulatory subunit Cdc55p and either catalytic subunit Pph21p or Pph22p; required for cell morphogenesis and transcription by RNA polymerase III (1, 2)
Name Description	tRNA Processing Deficient 3

Experimental Data
Molecules/cell	16900 4

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	635
Molecular Weight (Da)	70,906
Isoelectric Point (pI)	4.38

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Tpd3p (InterPro)
Physical Interactions	There are 113 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI00000531BD \| P31383 MIPS: YAL016W NCBI: 1345601 \| 171859 \| 173006 \| 308153639 \| 6319303 \| NP_009386.1 GenBank/EMBL/DDBJ: DAA06972.1 \| L05146 \| M98389

Amino Acid Sequence (or in FASTA format)
1 MSGARSTTAG AVPSAATTST TSTTSNSKDS DSNESLYPLA LLMDELKHDD 51 IANRVEAMKK LDTIALALGP ERTRNELIPF LTEVAQDDED EVFAVLAEQL 101 GKFVPYIGGP QYATILLPVL EILASAEETL VREKAVDSLN NVAQELSQEQ 151 LFSDFVPLIE HLATADWFSS KVSACGLFKS VIVRIKDDSL RKNILALYLQ 201 LAQDDTPMVK RAVGKNLPIL IDLLTQNLGL STDEDWDYIS NIFQKIINDN 251 QDSVKFLAVD CLISILKFFN AKGDESHTQD LLNSAVKLIG DEAWRVRYMA 301 ADRFSDLASQ FSSNQAYIDE LVQPFLNLCE DNEGDVREAV AKQVSGFAKF 351 LNDPSIILNK ILPAVQNLSM DESETVRSAL ASKITNIVLL LNKDQVINNF 401 LPILLNMLRD EFPDVRLNII ASLKVVNDVI GIELLSDSLL PAITELAKDV 451 NWRVRMAIIE YIPILAEQLG MQFFDQQLSD LCLSWLWDTV YSIREAAVNN 501 LKRLTEIFGS DWCRDEIISR LLKFDLQLLE NFVSRFTILS ALTTLVPVVS 551 LDVVTEQLLP FISHLADDGV PNIRFNVAKS YAVIVKVLIK DEAKYDALIK 601 NTILPSLQTL CQDEDVDVKY FAKKSLAECQ ELLKN*

Amino Acid Sequence (or in FASTA format)

       1  MSGARSTTAG AVPSAATTST TSTTSNSKDS DSNESLYPLA LLMDELKHDD
      51  IANRVEAMKK LDTIALALGP ERTRNELIPF LTEVAQDDED EVFAVLAEQL
     101  GKFVPYIGGP QYATILLPVL EILASAEETL VREKAVDSLN NVAQELSQEQ
     151  LFSDFVPLIE HLATADWFSS KVSACGLFKS VIVRIKDDSL RKNILALYLQ
     201  LAQDDTPMVK RAVGKNLPIL IDLLTQNLGL STDEDWDYIS NIFQKIINDN
     251  QDSVKFLAVD CLISILKFFN AKGDESHTQD LLNSAVKLIG DEAWRVRYMA
     301  ADRFSDLASQ FSSNQAYIDE LVQPFLNLCE DNEGDVREAV AKQVSGFAKF
     351  LNDPSIILNK ILPAVQNLSM DESETVRSAL ASKITNIVLL LNKDQVINNF
     401  LPILLNMLRD EFPDVRLNII ASLKVVNDVI GIELLSDSLL PAITELAKDV
     451  NWRVRMAIIE YIPILAEQLG MQFFDQQLSD LCLSWLWDTV YSIREAAVNN
     501  LKRLTEIFGS DWCRDEIISR LLKFDLQLLE NFVSRFTILS ALTTLVPVVS
     551  LDVVTEQLLP FISHLADDGV PNIRFNVAKS YAVIVKVLIK DEAKYDALIK
     601  NTILPSLQTL CQDEDVDVKY FAKKSLAECQ ELLKN*

external links for Tpd3p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Candida (CGD)	BioPIXIE	MIPS	YeastRC Public Image Repository
Candida (CandidaDB)	CYC2008 (complexes)	Pfam domains
YGOB	Complexome	YeastRC Structure Prediction (Seattle)
YOGY	DIP
	GeneMANIA

References cited on this page View Complete Literature Guide for Tpd3p

1)	van Zyl W, et al. (1992) Inactivation of the protein phosphatase 2A regulatory subunit A results in morphological and transcriptional defects in Saccharomyces cerevisiae. Mol Cell Biol 12(11):4946-59
2)	Wang Y and Burke DJ (1997) Cdc55p, the B-type regulatory subunit of protein phosphatase 2A, has multiple functions in mitosis and is required for the kinetochore/spindle checkpoint in Saccharomyces cerevisiae. Mol Cell Biol 17(2):620-6
3)	van Zyl WH, et al. (1989) A general screen for mutant of Saccharomyces cerevisiae deficient in tRNA biosynthesis. Genetics 123(1):55-68
4)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41