CYS3/YAL012W Protein Information

Standard Name	Cys3p
Systematic Name	Yal012wp
Alias	Cyi1p , Fun35p , Str1p 1
ORF Classification	Verified
Description	Cystathionine gamma-lyase, catalyzes one of the two reactions involved in the transsulfuration pathway that yields cysteine from homocysteine with the intermediary formation of cystathionine; protein abundance increases in response to DNA replication stress (2, 3, 4, 5)
Name Description	CYStathionine gamma-lyase

Experimental Data
Molecules/cell	38300 6

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	394
Molecular Weight (Da)	42,542
Isoelectric Point (pI)	6.52

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Cys3p (InterPro)
Physical Interactions	There are 16 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000005324D \| P31373 MIPS: YAL012W NCBI: 171863 \| 27066022 \| 27066023 \| 27066024 \| 27066025 \| 399331 \| 416164 \| 6319307 \| NP_009390.1 GenBank/EMBL/DDBJ: DAA06976.1 \| D14135 \| L05146
External Classifications	EC: 4.4.1.1 [Cystathionine gamma-lyase]

Amino Acid Sequence (or in FASTA format)
1 MTLQESDKFA TKAIHAGEHV DVHGSVIEPI SLSTTFKQSS PANPIGTYEY 51 SRSQNPNREN LERAVAALEN AQYGLAFSSG SATTATILQS LPQGSHAVSI 101 GDVYGGTHRY FTKVANAHGV ETSFTNDLLN DLPQLIKENT KLVWIETPTN 151 PTLKVTDIQK VADLIKKHAA GQDVILVVDN TFLSPYISNP LNFGADIVVH 201 SATKYINGHS DVVLGVLATN NKPLYERLQF LQNAIGAIPS PFDAWLTHRG 251 LKTLHLRVRQ AALSANKIAE FLAADKENVV AVNYPGLKTH PNYDVVLKQH 301 RDALGGGMIS FRIKGGAEAA SKFASSTRLF TLAESLGGIE SLLEVPAVMT 351 HGGIPKEARE ASGVFDDLVR ISVGIEDTDD LLEDIKQALK QATN*

Amino Acid Sequence (or in FASTA format)

       1  MTLQESDKFA TKAIHAGEHV DVHGSVIEPI SLSTTFKQSS PANPIGTYEY
      51  SRSQNPNREN LERAVAALEN AQYGLAFSSG SATTATILQS LPQGSHAVSI
     101  GDVYGGTHRY FTKVANAHGV ETSFTNDLLN DLPQLIKENT KLVWIETPTN
     151  PTLKVTDIQK VADLIKKHAA GQDVILVVDN TFLSPYISNP LNFGADIVVH
     201  SATKYINGHS DVVLGVLATN NKPLYERLQF LQNAIGAIPS PFDAWLTHRG
     251  LKTLHLRVRQ AALSANKIAE FLAADKENVV AVNYPGLKTH PNYDVVLKQH
     301  RDALGGGMIS FRIKGGAEAA SKFASSTRLF TLAESLGGIE SLLEVPAVMT
     351  HGGIPKEARE ASGVFDDLVR ISVGIEDTDD LLEDIKQALK QATN*

external links for Cys3p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastGFP
Candida (CGD)	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
Candida (CandidaDB)	Complexome	YeastRC Structure Prediction (Seattle)
YGOB	DIP
YOGY	GeneMANIA
	YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Cys3p

1)	Cherest H and Surdin-Kerjan Y (1992) Genetic analysis of a new mutation conferring cysteine auxotrophy in Saccharomyces cerevisiae: updating of the sulfur metabolism pathway. Genetics 130(1):51-8
2)	Ono B, et al. (1991) Regulation of cystathionine gamma-lyase in Saccharomyces cerevisiae. Yeast 7(8):843-8
3)	Ono B, et al. (1992) Cloning and characterization of the CYS3 (CYI1) gene of Saccharomyces cerevisiae. J Bacteriol 174(10):3339-47
4)	Cherest H, et al. (1993) Cysteine biosynthesis in Saccharomyces cerevisiae occurs through the transsulfuration pathway which has been built up by enzyme recruitment. J Bacteriol 175(17):5366-74
5)	Tkach JM, et al. (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
6)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41