SSA1/YAL005C Protein Information 
| Standard Name | Ssa1p 1 |
|---|---|
| Systematic Name | Yal005cp |
| Alias | Yg100p 2 |
| ORF Classification | Verified |
| Description | ATPase involved in protein folding and NLS-directed nuclear transport; member of HSP70 family; forms chaperone complex with Ydj1p; localized to nucleus, cytoplasm, and cell wall; 98% identical with paralog Ssa2p, but subtle differences between the two proteins provide functional specificity with respect to propagation of yeast [URE3] prions and vacuolar-mediated degradations of gluconeogenesis enzymes; general targeting factor of Hsp104p to prion fibrils (3, 4, 5, 6, 7, 8, 9) |
| Name Description | Stress-Seventy subfamily A |
| Experimental Data | |
|---|---|
| Molecules/cell | 269000 10 |
| Click on image for expanded interactive view |
|---|
| Post-translational Modifications | PhosphoGRID | PhosphoPep Database |
|---|---|
| Domains/motifs | See the graphical view and list of proteins that share domains/motifs in common with Ssa1p (InterPro) |
| Physical Interactions | There are 752 total physical interactions (BioGRID) |
| Homologs | PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer |
| External Sequence Databases |
EBI: UPI0000168E53 | P10591 MIPS: YAL005C NCBI: 144228166 | 315364443 | 349747 | 417149 | NP_009396.2 GenBank/EMBL/DDBJ: DAA06982.1 | L22015 | X12926 |
external links for Ssa1p
| Homologs | Interaction Resources | Protein databases/Other | Localization Resources |
|---|---|---|---|
| BLASTP (NCBI) | BioGRID | SCOP Superfamily | Organelle DB |
| Ashbya (AGD) | BOND | GPMdb (Mass Spec.) | YPL+ |
| Aspergillus (AspGD) | BioPIXIE | MIPS | YeastGFP |
| YGOB | CYC2008 (complexes) | Pfam domains | |
| YOGY | Complexome | YeastRC Structure Prediction (Seattle) | |
| DIP | |||
| GeneMANIA |
References cited on this page View Complete Literature Guide for Ssa1p
| 1) | Slater, M. (1989) Personal Communication, Mortimer Map Edition 10 |
| 2) | Ingolia TD, et al. (1982) Saccharomyces cerevisiae contains a complex multigene family related to the major heat shock-inducible gene of Drosophila. Mol Cell Biol 2(11):1388-98 |
| 3) | Lopez-Ribot JL and Chaffin WL (1996) Members of the Hsp70 family of proteins in the cell wall of Saccharomyces cerevisiae. J Bacteriol 178(15):4724-6 |
| 4) | Kim S, et al. (1998) Folding in vivo of a newly translated yeast cytosolic enzyme is mediated by the SSA class of cytosolic yeast Hsp70 proteins. Proc Natl Acad Sci U S A 95(22):12860-5 |
| 5) | Shulga N, et al. (1999) A nuclear export signal prevents Saccharomyces cerevisiae Hsp70 Ssb1p from stimulating nuclear localization signal-directed nuclear transport. J Biol Chem 274(23):16501-7 |
| 6) | Ziegelhoffer T, et al. (1995) The dissociation of ATP from hsp70 of Saccharomyces cerevisiae is stimulated by both Ydj1p and peptide substrates. J Biol Chem 270(18):10412-9 |
| 7) | Bush GL and Meyer DI (1996) The refolding activity of the yeast heat shock proteins Ssa1 and Ssa2 defines their role in protein translocation. J Cell Biol 135(5):1229-37 |
| 8) | Sharma D and Masison DC (2011) Single methyl group determines prion propagation and protein degradation activities of yeast heat shock protein (Hsp)-70 chaperones Ssa1p and Ssa2p. Proc Natl Acad Sci U S A 108(33):13665-70 |
| 9) | Winkler J, et al. (2012) Hsp70 targets Hsp100 chaperones to substrates for protein disaggregation and prion fragmentation. J Cell Biol 198(3):387-404 |
| 10) | Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41 |
