TFC3/YAL001C Protein Information

Standard Name	Tfc3p 1
Systematic Name	Yal001cp
Alias	Fun24p 2 , Tsv115p 3, 4
ORF Classification	Verified
Description	Largest of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauB domain of TFIIIC that binds DNA at the BoxB promoter sites of tRNA and similar genes; cooperates with Tfc6p in DNA binding (1, 5, 6, 7)
Name Description	Transcription Factor class C 1
Gene Product	tau 138 1

Experimental Data
Molecules/cell	125 8

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	1,160
Molecular Weight (Da)	132,107
Isoelectric Point (pI)	10.16

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Tfc3p (InterPro)
Physical Interactions	There are 39 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000052E26 \| P34111 MIPS: YAL001C NCBI: 170944 \| 385245 \| 464875 \| 6319317 \| NP_009400.1 \| NM_001178148.1 GenBank/EMBL/DDBJ: DAA06985.1 \| L22015 \| M98261

Amino Acid Sequence (or in FASTA format)
1 MVLTIYPDEL VQIVSDKIAS NKGKITLNQL WDISGKYFDL SDKKVKQFVL 51 SCVILKKDIE VYCDGAITTK NVTDIIGDAN HSYSVGITED SLWTLLTGYT 101 KKESTIGNSA FELLLEVAKS GEKGINTMDL AQVTGQDPRS VTGRIKKINH 151 LLTSSQLIYK GHVVKQLKLK KFSHDGVDSN PYINIRDHLA TIVEVVKRSK 201 NGIRQIIDLK RELKFDKEKR LSKAFIAAIA WLDEKEYLKK VLVVSPKNPA 251 IKIRCVKYVK DIPDSKGSPS FEYDSNSADE DSVSDSKAAF EDEDLVEGLD 301 NFNATDLLQN QGLVMEEKED AVKNEVLLNR FYPLQNQTYD IADKSGLKGI 351 STMDVVNRIT GKEFQRAFTK SSEYYLESVD KQKENTGGYR LFRIYDFEGK 401 KKFFRLFTAQ NFQKLTNAED EISVPKGFDE LGKSRTDLKT LNEDNFVALN 451 NTVRFTTDSD GQDIFFWHGE LKIPPNSKKT PNKNKRKRQV KNSTNASVAG 501 NISNPKRIKL EQHVSTAQEP KSAEDSPSSN GGTVVKGKVV NFGGFSARSL 551 RSLQRQRAIL KVMNTIGGVA YLREQFYESV SKYMGSTTTL DKKTVRGDVD 601 LMVESEKLGA RTEPVSGRKI IFLPTVGEDA IQRYILKEKD SKKATFTDVI 651 HDTEIYFFDQ TEKNRFHRGK KSVERIRKFQ NRQKNAKIKA SDDAISKKST 701 SVNVSDGKIK RRDKKVSAGR TTVVVENTKE DKTVYHAGTK DGVQALIRAV 751 VVTKSIKNEI MWDKITKLFP NNSLDNLKKK WTARRVRMGH SGWRAYVDKW 801 KKMLVLAIKS EKISLRDVEE LDLIKLLDIW TSFDEKEIKR PLFLYKNYEE 851 NRKKFTLVRD DTLTHSGNDL AMSSMIQREI SSLKKTYTRK ISASTKDLSK 901 SQSDDYIRTV IRSILIESPS TTRNEIEALK NVGNESIDNV IMDMAKEKQI 951 YLHGSKLECT DTLPDILENR GNYKDFGVAF QYRCKVNELL EAGNAIVINQ 1001 EPSDISSWVL IDLISGELLN MDVIPMVRNV RPLTYTSRRF EIRTLTPPLI 1051 IYANSQTKLN TARKSAVKVP LGKPFSRLWV NGSGSIRPNI WKQVVTMVVN 1101 EIIFHPGITL SRLQSRCREV LSLHEISEIC KWLLERQVLI TTDFDGYWVN 1151 HNWYSIYEST *

Amino Acid Sequence (or in FASTA format)

       1  MVLTIYPDEL VQIVSDKIAS NKGKITLNQL WDISGKYFDL SDKKVKQFVL
      51  SCVILKKDIE VYCDGAITTK NVTDIIGDAN HSYSVGITED SLWTLLTGYT
     101  KKESTIGNSA FELLLEVAKS GEKGINTMDL AQVTGQDPRS VTGRIKKINH
     151  LLTSSQLIYK GHVVKQLKLK KFSHDGVDSN PYINIRDHLA TIVEVVKRSK
     201  NGIRQIIDLK RELKFDKEKR LSKAFIAAIA WLDEKEYLKK VLVVSPKNPA
     251  IKIRCVKYVK DIPDSKGSPS FEYDSNSADE DSVSDSKAAF EDEDLVEGLD
     301  NFNATDLLQN QGLVMEEKED AVKNEVLLNR FYPLQNQTYD IADKSGLKGI
     351  STMDVVNRIT GKEFQRAFTK SSEYYLESVD KQKENTGGYR LFRIYDFEGK
     401  KKFFRLFTAQ NFQKLTNAED EISVPKGFDE LGKSRTDLKT LNEDNFVALN
     451  NTVRFTTDSD GQDIFFWHGE LKIPPNSKKT PNKNKRKRQV KNSTNASVAG
     501  NISNPKRIKL EQHVSTAQEP KSAEDSPSSN GGTVVKGKVV NFGGFSARSL
     551  RSLQRQRAIL KVMNTIGGVA YLREQFYESV SKYMGSTTTL DKKTVRGDVD
     601  LMVESEKLGA RTEPVSGRKI IFLPTVGEDA IQRYILKEKD SKKATFTDVI
     651  HDTEIYFFDQ TEKNRFHRGK KSVERIRKFQ NRQKNAKIKA SDDAISKKST
     701  SVNVSDGKIK RRDKKVSAGR TTVVVENTKE DKTVYHAGTK DGVQALIRAV
     751  VVTKSIKNEI MWDKITKLFP NNSLDNLKKK WTARRVRMGH SGWRAYVDKW
     801  KKMLVLAIKS EKISLRDVEE LDLIKLLDIW TSFDEKEIKR PLFLYKNYEE
     851  NRKKFTLVRD DTLTHSGNDL AMSSMIQREI SSLKKTYTRK ISASTKDLSK
     901  SQSDDYIRTV IRSILIESPS TTRNEIEALK NVGNESIDNV IMDMAKEKQI
     951  YLHGSKLECT DTLPDILENR GNYKDFGVAF QYRCKVNELL EAGNAIVINQ
    1001  EPSDISSWVL IDLISGELLN MDVIPMVRNV RPLTYTSRRF EIRTLTPPLI
    1051  IYANSQTKLN TARKSAVKVP LGKPFSRLWV NGSGSIRPNI WKQVVTMVVN
    1101  EIIFHPGITL SRLQSRCREV LSLHEISEIC KWLLERQVLI TTDFDGYWVN
    1151  HNWYSIYEST *

external links for Tfc3p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Candida (CGD)	BioPIXIE	MIPS	YeastRC Public Image Repository
Candida (CandidaDB)	CYC2008 (complexes)	Pfam domains
YGOB	Complexome	YeastRC Structure Prediction (Seattle)
YOGY	DIP
	GeneMANIA

References cited on this page View Complete Literature Guide for Tfc3p

1)	Lefebvre O, et al. (1992) TFC3: gene encoding the B-block binding subunit of the yeast transcription factor IIIC. Proc Natl Acad Sci U S A 89(21):10512-6
2)	Clark MW, et al. (1994) Sequencing of chromosome I of Saccharomyces cerevisiae: analysis of the 42 kbp SPO7-CENI-CDC15 region. Yeast 10(4):535-41
3)	Lefebvre O, et al. (1994) A mutation in the largest subunit of yeast TFIIIC affects tRNA and 5 S RNA synthesis. Identification of two classes of suppressors. J Biol Chem 269(37):23374-81
4)	Harris SD and Pringle JR (1991) Genetic analysis of Saccharomyces cerevisiae chromosome I: on the role of mutagen specificity in delimiting the set of genes identifiable using temperature-sensitive-lethal mutations. Genetics 127(2):279-85
5)	Conesa C, et al. (1993) On the subunit composition, stoichiometry, and phosphorylation of the yeast transcription factor TFIIIC/tau. J Biol Chem 268(24):18047-52
6)	Geiduschek EP and Kassavetis GA (2001) The RNA polymerase III transcription apparatus. J Mol Biol 310(1):1-26
7)	Schultz P, et al. (1989) The two DNA-binding domains of yeast transcription factor tau as observed by scanning transmission electron microscopy. EMBO J 8(12):3815-24
8)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41