CEN1 Locus History Help

Sequence Annotation Notes
DateNote
2004-10-12Centromeric DNA elements CDEI, CDEII, and CDEIII were annotated based on Wieland et al. 2001 and Espelin et al. 2003.

Wieland G, et al.  (2001) Determination of the binding constants of the centromere protein Cbf1 to all 16 centromere DNAs of Saccharomyces cerevisiae. Nucleic Acids Res 29(5):1054-60
Espelin CW, et al.  (2003) Binding of the essential Saccharomyces cerevisiae kinetochore protein Ndc10p to CDEII. Mol Biol Cell 14(11):4557-68
2006-05-08The previously annotated boundaries of CEN1 were adjusted to coincide with the 5' end of CDEI and the 3' end of CDEIII, to more accurately reflect current knowledge regarding centromere structure in Saccharomyces cerevisiae.

Wieland G, et al.  (2001) Determination of the binding constants of the centromere protein Cbf1 to all 16 centromere DNAs of Saccharomyces cerevisiae. Nucleic Acids Res 29(5):1054-60
Espelin CW, et al.  (2003) Binding of the essential Saccharomyces cerevisiae kinetochore protein Ndc10p to CDEII. Mol Biol Cell 14(11):4557-68
2011-02-03A dinucleotide substitution was made in the intergenic region between CEN1 and ORF NUP60/YAR002W 
New    152153  ATATATTTACAAGTGAAAGCTTATTGTAATGTGTACTTTTAAACATCAAATAACAGACCT  152212
               ||||||||||||||||||||||||||||||||||  ||||||||||||||||||||||||
Old    152155  ATATATTTACAAGTGAAAGCTTATTGTAATGTGTCATTTTAAACATCAAATAACAGACCT  152214
Dietrich F  (2011) Personal Communication, An updated S. cerevisiae reference genome: Resequencing of S288C using NextGen sequencing technology. ()
Mapping Notes
DateNote
1996-09-21Edition 13: There are three conserved sequence elements in the centromere that are sufficient for mitotic and meiotic segregation. CDEI (8bp) is required for mitotic segregation; although most assays indicate it is dispensable for meiotic chromosome segregation, it is needed for meiosis I plasmid disjunction. CDEII (78-86 bp A-T rich region) is specifically required for meiosis I disjunction. CDEIII (25 bp) is essential for both mitotic and meiotic segregation.

Cherry JM, et al.  (1996) "Genetic and Physical Maps of Saccharomyces cerevisiae (Edition 13)". Pp. 361-364 in 1996 Yeast Genetics and Molecular Biology Meeting Program and Abstracts. Bethesda, MD: The Genetics Society of America