CEN7 Locus History

Nomenclature History
Standard Name	Reference
CEN7	Balzi, E. (1989) Personal Communication, Mortimer Map Edition 10

Sequence Annotation Notes
Date	Note
2004-10-12	The orientation of this centromere was reversed (from Watson to Crick) and its coordinates expanded to accommodate annotation of the centromeric DNA elements CDEI, CDEII, and CDEIII based on Wieland et al. (2001) and Espelin et al. (2003).
	Wieland G, et al. (2001) Determination of the binding constants of the centromere protein Cbf1 to all 16 centromere DNAs of Saccharomyces cerevisiae. Nucleic Acids Res 29(5):1054-60
	Espelin CW, et al. (2003) Binding of the essential Saccharomyces cerevisiae kinetochore protein Ndc10p to CDEII. Mol Biol Cell 14(11):4557-68
2006-05-09	The previously annotated 3' boundary of CEN7 was moved 1 bp upstream to coincide with the 3' end of CDEIII, to more accurately reflect current knowledge regarding centromere structure in Saccharomyces cerevisiae.
	Wieland G, et al. (2001) Determination of the binding constants of the centromere protein Cbf1 to all 16 centromere DNAs of Saccharomyces cerevisiae. Nucleic Acids Res 29(5):1054-60
	Espelin CW, et al. (2003) Binding of the essential Saccharomyces cerevisiae kinetochore protein Ndc10p to CDEII. Mol Biol Cell 14(11):4557-68

Mapping Notes
Date	Note
1989-10-01	Edition 10: cen7-pma1 physical distance is 35 kb
	Mortimer RK, et al. (1989) Genetic map of Saccharomyces cerevisiae, edition 10. Yeast 5(5):321-403
1996-09-21	Edition 13: There are three conserved sequence elements in the centromere that are sufficient for mitotic and meiotic segregation. CDEI (8bp) is required for mitotic segregation; although most assays indicate it is dispensable for meiotic chromosome segregation, it is needed for meiosis I plasmid disjunction. CDEII (78-86 bp A-T rich region) is specifically required for meiosis I disjunction. CDEIII (25 bp) is essential for both mitotic and meiotic segregation.
	Cherry JM, et al. (1996) "Genetic and Physical Maps of Saccharomyces cerevisiae (Edition 13)". Pp. 361-364 in 1996 Yeast Genetics and Molecular Biology Meeting Program and Abstracts. Bethesda, MD: The Genetics Society of America