| 1997-10-20 | Edition 14: SOP10 was pulled out of a yeast genomic library by a two-hybrid screen with Sec1p. The clone that binds Sec1p encodes the 401 C-terminal amino acids of YLR425W. No in-vitro binding to Sec1p could be detected by either immunoprecipitation from yeast lysates or by immunoprecipitation from in vitro transcription-translation products of SOP1 and SEC1. Haploids and diploids deleted for SOP1 appear to be clustered and have multiple buds, but the budding pattern appears fairly normal by chitin staining. Actin cables appear intact by phalloidin staining. Fluid phase endocytosis is normal, by lucifer yellow uptake. Cells appear wild-type by electron microscopy, and there is no block in invertase secretion. The sequence of SOP1 bears homology to the Rho1p GDP/GTP exchange factors, ROM1 and ROM2. This homology is in the conserved, GRF-DBL domain.
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