SMT3/YDR510W Summary Help

Standard Name SMT3
Systematic Name YDR510W
Feature Type ORF, Verified
Description Ubiquitin-like protein of the SUMO family; conjugated to lysine residues of target proteins; associates with transcriptionally active genes; regulates chromatid cohesion, chromosome segregation, APC-mediated proteolysis, DNA replication and septin ring dynamics; phosphorylated at Ser2 (1, 2, 3, 4, 5, 6, 7, 8, 9)
Name Description Suppressor of Mif Two
Chromosomal Location
ChrIV:1469400 to 1469705 | ORF Map | GBrowse
Gene Ontology Annotations All SMT3 GO evidence and references
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 21 genes
Classical genetics
dominant negative
Large-scale survey
reduction of function
1938 total interaction(s) for 1011 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 1016
  • Affinity Capture-RNA: 4
  • Affinity Capture-Western: 187
  • Biochemical Activity: 17
  • Co-crystal Structure: 4
  • Co-localization: 1
  • Co-purification: 2
  • PCA: 375
  • Protein-peptide: 3
  • Reconstituted Complex: 21
  • Two-hybrid: 101

Genetic Interactions
  • Dosage Growth Defect: 3
  • Dosage Rescue: 5
  • Negative Genetic: 31
  • Phenotypic Suppression: 6
  • Positive Genetic: 6
  • Synthetic Growth Defect: 144
  • Synthetic Lethality: 7
  • Synthetic Rescue: 5

Expression Summary
Length (a.a.) 101
Molecular Weight (Da) 11,597
Isoelectric Point (pI) 4.76
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrIV:1469400 to 1469705 | ORF Map | GBrowse
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..306 1469400..1469705 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002918
References cited on this page View Complete Literature Guide for SMT3
1) Johnson ES and Blobel G  (1999) Cell cycle-regulated attachment of the ubiquitin-related protein SUMO to the yeast septins. J Cell Biol 147(5):981-94
2) Takahashi Y, et al.  (1999) Smt3, a SUMO-1 homolog, is conjugated to Cdc3, a component of septin rings at the mother-bud neck in budding yeast. Biochem Biophys Res Commun 259(3):582-7
3) Biggins S, et al.  (2001) Genes involved in sister chromatid separation and segregation in the budding yeast Saccharomyces cerevisiae. Genetics 159(2):453-70
4) Bachant J, et al.  (2002) The SUMO-1 isopeptidase Smt4 is linked to centromeric cohesion through SUMO-1 modification of DNA topoisomerase II. Mol Cell 9(6):1169-82
5) Hoege C, et al.  (2002) RAD6-dependent DNA repair is linked to modification of PCNA by ubiquitin and SUMO. Nature 419(6903):135-41
6) Dieckhoff P, et al.  (2004) Smt3/SUMO and Ubc9 are required for efficient APC/C-mediated proteolysis in budding yeast. Mol Microbiol 51(5):1375-87
7) Stead K, et al.  (2003) Pds5p regulates the maintenance of sister chromatid cohesion and is sumoylated to promote the dissolution of cohesion. J Cell Biol 163(4):729-41
8) Matic I, et al.  (2008) Phosphorylation of SUMO-1 Occurs in Vivo and Is Conserved through Evolution. J Proteome Res 7(9):4050-4057
9) Rosonina E, et al.  (2010) SUMO functions in constitutive transcription and during activation of inducible genes in yeast. Genes Dev 24(12):1242-52