SWI5/YDR146C Summary Help

Standard Name SWI5 1, 2
Systematic Name YDR146C
Feature Type ORF, Verified
Description Transcription factor that recruits Mediator and Swi/Snf complexes; activates transcription of genes expressed at the M/G1 phase boundary and in G1 phase; required for expression of the HO gene controlling mating type switching; localization to nucleus occurs during G1 and appears to be regulated by phosphorylation by Cdc28p kinase; SWI5 has a paralog, ACE2, that arose from the whole genome duplication (1, 3, 4, 5, 6, 7, 8, 9 and see Summary Paragraph)
Name Description SWItching deficient 1
Chromosomal Location
ChrIV:750742 to 748613 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gene Ontology Annotations All SWI5 GO evidence and references
  View Computational GO annotations for SWI5
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Targets 127 genes
Regulators 9 genes
Classical genetics
Large-scale survey
294 total interaction(s) for 224 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 15
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 7
  • Biochemical Activity: 5
  • Co-localization: 7
  • PCA: 1
  • Reconstituted Complex: 10
  • Two-hybrid: 16

Genetic Interactions
  • Dosage Growth Defect: 2
  • Dosage Lethality: 3
  • Dosage Rescue: 5
  • Negative Genetic: 128
  • Phenotypic Enhancement: 6
  • Phenotypic Suppression: 5
  • Positive Genetic: 43
  • Synthetic Growth Defect: 19
  • Synthetic Lethality: 5
  • Synthetic Rescue: 16

Expression Summary
Length (a.a.) 709
Molecular Weight (Da) 79,774
Isoelectric Point (pI) 9.11
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrIV:750742 to 748613 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..2130 750742..748613 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002553

SWI5 encodes a transcription factor that activates transcription of genes expressed at the M/G1 boundary and in G1 phase of the cell cycle (6, 10, 5). The DNA binding domain of Swi5p is very similar to that of another G1-specific transcription activator, Ace2p (6). Swi5p and Ace2p bind the same DNA sequences in vitro with similar affinites, and regulate a shared set of genes in vivo (10, 5); however, the two proteins also show distinct promoter specificities in some instances (6, 5). For example, Swi5p activates transcription of the HO endonuclease gene, whereas Ace2p does not; conversely, CTS1 is activated by Ace2p and not by Swi5p (6). Distinct regions of Swi5p and Ace2p that are involved in promoter-specific transcription activation have been identified (5).

SWI5 is transcribed in G2 phase (11); cell cycle specific SWI5 expression requires Mcm1p and the uncloned "Swi Five Factor (SFF)" (12, 13). The localization of Swi5p is regulated by phosphorylation; dephosphorylated Swi5p is found in the nucleus, and therefore active, early in G1 phase (14, 3).

Last updated: 1999-12-03 Contact SGD

References cited on this page View Complete Literature Guide for SWI5
1) Stern M, et al.  (1984) Five SWI genes are required for expression of the HO gene in yeast. J Mol Biol 178(4):853-68
2) Stillman, D.  (1993) Personal Communication, Mortimer Map Edition 12
3) Moll T, et al.  (1991) The role of phosphorylation and the CDC28 protein kinase in cell cycle-regulated nuclear import of the S. cerevisiae transcription factor SWI5. Cell 66(4):743-58
4) Cosma MP, et al.  (1999) Ordered recruitment of transcription and chromatin remodeling factors to a cell cycle- and developmentally regulated promoter. Cell 97(3):299-311
5) McBride HJ, et al.  (1999) Distinct regions of the Swi5 and Ace2 transcription factors are required for specific gene activation. J Biol Chem 274(30):21029-36
6) Dohrmann PR, et al.  (1992) Parallel pathways of gene regulation: homologous regulators SWI5 and ACE2 differentially control transcription of HO and chitinase. Genes Dev 6(1):93-104
7) Bhoite LT, et al.  (2001) The Swi5 activator recruits the Mediator complex to the HO promoter without RNA polymerase II. Genes Dev 15(18):2457-69
8) Ubersax JA, et al.  (2003) Targets of the cyclin-dependent kinase Cdk1. Nature 425(6960):859-64
9) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
10) Dohrmann PR, et al.  (1996) Role of negative regulation in promoter specificity of the homologous transcriptional activators Ace2p and Swi5p. Mol Cell Biol 16(4):1746-58
11) Nasmyth K, et al.  (1987) Cell cycle regulation of SW15 is required for mother-cell-specific HO transcription in yeast. Cell 49(4):549-58
12) Lydall D, et al.  (1991) A new role for MCM1 in yeast: cell cycle regulation of SW15 transcription. Genes Dev 5(12B):2405-19
13) Althoefer H, et al.  (1995) Mcm1 is required to coordinate G2-specific transcription in Saccharomyces cerevisiae. Mol Cell Biol 15(11):5917-28
14) Nasmyth K, et al.  (1990) The identification of a second cell cycle control on the HO promoter in yeast: cell cycle regulation of SW15 nuclear entry. Cell 62(4):631-47
15) Badis G, et al.  (2008) A library of yeast transcription factor motifs reveals a widespread function for Rsc3 in targeting nucleosome exclusion at promoters. Mol Cell 32(6):878-87
16) Zhu J and Zhang MQ  (1999) SCPD: a promoter database of the yeast Saccharomyces cerevisiae. Bioinformatics 15(7-8):607-11