NUP120/YKL057C Summary Help

Standard Name NUP120 1
Systematic Name YKL057C
Alias RAT2 2
Feature Type ORF, Verified
Description Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis and is involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP160 (1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11 and see Summary Paragraph)
Name Description NUclear Pore 1
Chromosomal Location
ChrXI:333969 to 330856 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All NUP120 GO evidence and references
  View Computational GO annotations for NUP120
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 1 genes
Resources
Classical genetics
null
Large-scale survey
null
Resources
309 total interaction(s) for 203 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 64
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 7
  • Co-localization: 1
  • Co-purification: 4
  • FRET: 5
  • PCA: 6
  • Reconstituted Complex: 5
  • Two-hybrid: 3

Genetic Interactions
  • Dosage Growth Defect: 2
  • Dosage Lethality: 2
  • Dosage Rescue: 1
  • Negative Genetic: 112
  • Phenotypic Enhancement: 2
  • Positive Genetic: 28
  • Synthetic Growth Defect: 10
  • Synthetic Lethality: 55
  • Synthetic Rescue: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 1,037
Molecular Weight (Da) 120,447
Isoelectric Point (pI) 4.91
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXI:333969 to 330856 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..3114 333969..330856 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001540
SUMMARY PARAGRAPH for NUP120

NUP120 encodes a non-essential nuclear pore protein (1, 2). Transport of macromolecules between the nucleus and the cytoplasm of eukaryotic cells occurs through the nuclear pore complex (NPC), a large macromolecular complex that spans the nuclear envelope (reviewed in 12, 13, 14, 15). The structure of the vertebrate NPC has been studied extensively; recent reviews include 16, 17, 18, and 19. The yeast NPC shares several features with the vertebrate NPC, despite being smaller and less elaborate (20, 21). Many yeast nuclear pore proteins, or nucleoporins, have been identified by a variety of genetic approaches (reviewed in 12, 13, 22, 23, 24). Nup120p is part of subcomplex within the NPC that also includes Sec13p, Seh1p, Nup84p, and Nup85p (25, 12). PH, A nup120 mutation is synthetically lethal with mutations in NUP133 and NUP159, which also encode nuclear pore proteins. Also, Nup85p cannot be detected in nup120 mutants (26).

Last updated: 1999-08-09 Contact SGD

References cited on this page View Complete Literature Guide for NUP120
1) Aitchison JD, et al.  (1995) Nup120p: a yeast nucleoporin required for NPC distribution and mRNA transport. J Cell Biol 131(6 Pt 2):1659-75
2) Heath CV, et al.  (1995) Nuclear pore complex clustering and nuclear accumulation of poly(A)+ RNA associated with mutation of the Saccharomyces cerevisiae RAT2/NUP120 gene. J Cell Biol 131(6 Pt 2):1677-97
3) Siniossoglou S, et al.  (2000) Structure and assembly of the Nup84p complex. J Cell Biol 149(1):41-54
4) Rout MP, et al.  (2000) The yeast nuclear pore complex: composition, architecture, and transport mechanism. J Cell Biol 148(4):635-51
5) Gao H, et al.  (2003) Nuclear accumulation of the small GTPase Gsp1p depends on nucleoporins Nup133p, Rat2p/Nup120p, Nup85p, Nic96p, and the acetyl-CoA carboxylase Acc1p. J Biol Chem 278(28):25331-40
6) Therizols P, et al.  (2006) Telomere tethering at the nuclear periphery is essential for efficient DNA double strand break repair in subtelomeric region. J Cell Biol 172(2):189-99
7) Lutzmann M, et al.  (2002) Modular self-assembly of a Y-shaped multiprotein complex from seven nucleoporins. EMBO J 21(3):387-97
8) Fernandez-Martinez J and Rout MP  (2009) Nuclear pore complex biogenesis. Curr Opin Cell Biol 21(4):603-12
9) Tous C, et al.  (2011) A novel assay identifies transcript elongation roles for the Nup84 complex and RNA processing factors. EMBO J 30(10):1953-64
10) Sarma NJ, et al.  (2011) The nuclear pore complex mediates binding of the mig1 repressor to target promoters. PLoS One 6(11):e27117
11) Fernandez-Martinez J, et al.  (2012) Structure-function mapping of a heptameric module in the nuclear pore complex. J Cell Biol 196(4):419-34
12) Fabre E and Hurt E  (1997) Yeast genetics to dissect the nuclear pore complex and nucleocytoplasmic trafficking. Annu Rev Genet 31:277-313
13) Wente SR, et al.  (1997) "The nucleus and nucleocytoplasmic transport in Saccharomyces cerevisiae." Pp. 471-546 in The Molecular and Cellular Biology of the Yeast Saccharomyces: Cell Cycle and Cell Biology, edited by Pringle JR, Broach JR and Jones EW. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press
14) Pemberton LF, et al.  (1998) Transport routes through the nuclear pore complex. Curr Opin Cell Biol 10(3):392-9
15) Izaurralde E and Adam S  (1998) Transport of macromolecules between the nucleus and the cytoplasm. RNA 4(4):351-64
16) Hinshaw JE  (1994) Architecture of the nuclear pore complex and its involvement in nucleocytoplasmic transport. Biochem Pharmacol 47(1):15-20
17) Pante N and Aebi U  (1996) Molecular dissection of the nuclear pore complex. Crit Rev Biochem Mol Biol 31(2):153-99
18) Davis LI  (1995) The nuclear pore complex. Annu Rev Biochem 64:865-96
19) Pante N and Aebi U  (1994) Toward the molecular details of the nuclear pore complex. J Struct Biol 113(3):179-89
20) Rout MP and Blobel G  (1993) Isolation of the yeast nuclear pore complex. J Cell Biol 123(4):771-83
21) Yang Q, et al.  (1998) Three-dimensional architecture of the isolated yeast nuclear pore complex: functional and evolutionary implications. Mol Cell 1(2):223-34
22) Doye V and Hurt E  (1997) From nucleoporins to nuclear pore complexes. Curr Opin Cell Biol 9(3):401-11
23) Doye V and Hurt EC  (1995) Genetic approaches to nuclear pore structure and function. Trends Genet 11(6):235-41
24) Newmeyer DD  (1993) The nuclear pore complex and nucleocytoplasmic transport. Curr Opin Cell Biol 5(3):395-407
25) Siniossoglou S, et al.  (1996) A novel complex of nucleoporins, which includes Sec13p and a Sec13p homolog, is essential for normal nuclear pores. Cell 84(2):265-75
26) Goldstein AL, et al.  (1996) Pleiotropic nuclear defects associated with a conditional allele of the novel nucleoporin Rat9p/Nup85p. Mol Biol Cell 7(6):917-34