ATP8/Q0080 Summary Help

Standard Name ATP8
Systematic Name Q0080
Alias AAP1
Feature Type ORF, Verified
Description Subunit 8 of the F0 sector of mitochondrial F1F0 ATP synthase; encoded on the mitochondrial genome; ATP8 and ATP6 mRNAs are not translated in the absence of the F1 sector of ATPase (1, 2 and see Summary Paragraph)
Name Description ATP synthase
Chromosomal Location
ChrMito:27666 to 27812 | ORF Map | GBrowse
Gene Ontology Annotations All ATP8 GO evidence and references
  View Computational GO annotations for ATP8
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 2 genes
Classical genetics
reduction of function
16 total interaction(s) for 9 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 5
  • Affinity Capture-Western: 7
  • Co-purification: 3

Genetic Interactions
  • Phenotypic Suppression: 1

Expression Summary
Length (a.a.) 48
Molecular Weight (Da) 5,822
Isoelectric Point (pI) 10.89
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrMito:27666 to 27812 | ORF Map | GBrowse
Last Update Coordinates: 2000-05-19 | Sequence: 2000-05-19
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..147 27666..27812 2000-05-19 2000-05-19
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000007267

ATP8 is one of three mitochondrial genes, along with OLI1 and ATP6, that encode ATP synthase subunits (3). The ATP synthase complex utilizes proton motive force to generate ATP from ADP and Pi (4). The structure of this enzyme complex is highly conserved among diverse organisms and consists of two major components, soluble F1 and membrane-bound F0, each of which contains many subunits. Atp8p (also known as subunit 8 or Y8) is homologous to mammalian mitochondrial subunit A6L, but has no bacterial homolog. Although the specific function of this integral membrane protein is not yet known, it is essential for ATP synthase assembly and activity (1 and references therein). Deletion of ATP8, like deletions in many genes necessary for the function or maintenance of mitochondria, leads to a "petite" phenotype that is slow-growing and unable to survive on nonfermentable carbon sources (5).

General ATP synthase structure and function are reviewed in references 4 and 6. For a review that is specific to yeast, see reference 1.

Last updated: 2006-04-03 Contact SGD

References cited on this page View Complete Literature Guide for ATP8
1) Devenish RJ, et al.  (2000) Insights into ATP synthase assembly and function through the molecular genetic manipulation of subunits of the yeast mitochondrial enzyme complex. Biochim Biophys Acta 1458(2-3):428-42
2) Rak M and Tzagoloff A  (2009) F1-dependent translation of mitochondrially encoded Atp6p and Atp8p subunits of yeast ATP synthase. Proc Natl Acad Sci U S A 106(44):18509-14
3) Macreadie IG, et al.  (1983) Biogenesis of mitochondria: the mitochondrial gene (aap1) coding for mitochondrial ATPase subunit 8 in Saccharomyces cerevisiae. Nucleic Acids Res 11(13):4435-51
4) Boyer PD  (1997) The ATP synthase--a splendid molecular machine. Annu Rev Biochem 66:717-49
5) Marzuki S, et al.  (1989) Mitochondrial H+-ATPase in mutants of Saccharomyces cerevisiae with defective subunit 8 of the enzyme complex. Biochim Biophys Acta 975(2):222-30
6) Nakamoto RK, et al.  (1999) Rotational coupling in the F0F1 ATP synthase. Annu Rev Biophys Biomol Struct 28():205-34