SCR1 Summary Help

Standard Name SCR1
Feature Type ncRNA
Description RNA subunit of the Signal Recognition Particle (SRP) (1 and see Summary Paragraph)
Name Description Small Cytoplasmic RNA
Chromosomal Location
ChrV:441987 to 442508 | ORF Map | GBrowse
Gene Ontology Annotations All SCR1 GO evidence and references
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Classical genetics
1 total interaction(s) for 1 unique genes/features.
Physical Interactions
  • Affinity Capture-RNA: 1

sequence information
ChrV:441987 to 442508 | ORF Map | GBrowse
Last Update Coordinates: 2011-02-03 | Sequence: 2004-10-08
Subfeature details
Most Recent Updates
Coordinates Sequence
Noncoding exon 1..522 441987..442508 2011-02-03 2004-10-08
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
External Links All Associated Seq | Search all NCBI (Entrez)
Primary SGDIDS000006491

The signal recognition particle (SRP) is an abundant and conserved ribonucleoprotein necessary for targeting proteins to the endoplasmic reticulum membrane (2). SRP in eukaryotes contains six subunits and a 7S RNA molecule; in S. cerevisiae the subunits are encoded by SRP14, SRP21, SRP68, SRP72, SEC65, and SRP54, and the RNA (termed scR1) is encoded by SCR1 (2, 1). With the exception of Srp54p, the proteins and RNA assemble into a core complex in the nucleus; this particle is exported to the cytoplasm where Srp54p joins to form the complete complex (3). Sec65p is required for association of Srp54p with the SRP particle (4). Loss of any of the SRP components causes a slow growth phenotype and loss of SRP-mediated translocation, but not cell death, indicating that the signal recognition particle is not essential in yeast and SRP-independent translocation can occur (2, 1).

The first step of SRP-mediated cotranslational targeting is interaction between SRP and the ribosome nascent chain complex (RNC), which is comprised of the translating ribosome and the emerging nascent protein. SRP interacts with the RNC through the N-terminal hydrophobic signal sequence of the nascent protein. SRP then directs the RNC to the ER membrane via interaction between SRP and a signal receptor complex (SR), encoded by SRP101 and SRP102. Finally, the RNC is transferred to the translocon, a protein-conducting membrane channel, and SRP and the SR dissociate. GTP binding by both SRP (via the Srp54p subunit) and the SR is critical for their interaction, and GTP hydrolysis facilitates their dissociation (reviewed in 5, and see 5 for more details).

Last updated: 2008-08-11 Contact SGD

References cited on this page View Complete Literature Guide for SCR1
1) Brown JD, et al.  (1994) Subunits of the Saccharomyces cerevisiae signal recognition particle required for its functional expression. EMBO J 13(18):4390-400
2) Hann BC and Walter P  (1991) The signal recognition particle in S. cerevisiae. Cell 67(1):131-44
3) Ciufo LF and Brown JD  (2000) Nuclear export of yeast signal recognition particle lacking Srp54p by the Xpo1p/Crm1p NES-dependent pathway. Curr Biol 10(20):1256-64
4) Stirling CJ and Hewitt EW  (1992) The S. cerevisiae SEC65 gene encodes a component of yeast signal recognition particle with homology to human SRP19. Nature 356(6369):534-7
5) Wild K, et al.  (2004) SRP meets the ribosome. Nat Struct Mol Biol 11(11):1049-53