PSO2/YMR137C Summary

Standard Name	PSO2 1 (see Nomenclature conflict Note)
Systematic Name	YMR137C
Alias	SNM1 2
Feature Type	ORF, Verified
Description	Nuclease required for DNA single- and double-strand break repair; acts at a post-incision step in repair of breaks that result from interstrand cross-links produced by a variety of mono- and bi-functional psoralen derivatives; induced by UV-irradiation; forms nuclear foci upon DNA replication stress (3, 4, 5, 6, 7, 8, 9, 10)
Name Description	PSOralen derivative sensitive 1

Chromosomal Location	ChrXIII:544963 to 542978 \| ORF Map \| GBrowse
	Note: this feature is encoded on the Crick strand.

Gene Ontology Annotations All PSO2 GO evidence and references

	View Computational GO annotations for PSO2
Molecular Function
Manually curated	5'-3' exonuclease activity (IDA, IMP) damaged DNA binding (IMP)
Biological Process
Manually curated	DNA repair (IMP) double-strand break repair (TAS) interstrand cross-link repair (IGI, IMP)
Cellular Component
Manually curated	nucleus (IC)
High-throughput	nucleus (IDA)

Mutant phenotypes All PSO2 Phenotype evidence and references

Classical genetics
null	chromosome/plasmid maintenance: decreased resistance to nitrogen mustard: decreased resistance to mitomycin C: decreased resistance to oxaliplatin: decreased resistance to cisplatin: decreased
unspecified	mitochondrial rho- mutation frequency: increased mitochondrial rho- mutation frequency: decreased mutation frequency: decreased resistance to mechlorethamine: decreased resistance to 3-carbethoxypsoralen: decreased resistance to 8-methoxypsoralen: decreased
Large-scale survey
null	competitive fitness: decreased haploinsufficient resistance to selenomethionine: increased resistance to cisplatin: decreased resistance to cycloheximide: decreased resistance to amitrole: decreased viable
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All PSO2 Interaction evidence and references

	44 total interaction(s) for 30 unique genes/features.
Physical Interactions	Affinity Capture-MS: 4
Genetic Interactions	Negative Genetic: 5 Phenotypic Enhancement: 24 Phenotypic Suppression: 2 Positive Genetic: 2 Synthetic Growth Defect: 6 Synthetic Rescue: 1
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All PSO2 Protein evidence and references

Localization	YeastRC \| YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| CGD Homologs \| CandidaDB Homologs \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrXIII:544963 to 542978 | |

Note: this feature is encoded on the Crick strand.

Last Update

Coordinates: 2011-02-03 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..1986	544963..542978	2011-02-03	1996-07-31

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| E.C. \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000004745

NOMENCLATURE CONFLICT NOTE

Name	Relevance	Description
SNM1	Nomenclature conflict	SNM1 has been used to refer to both PSO2/YMR137C, which encodes a protein involved in DNA repair, and SNM1/YDR478W, which encodes a subunit of RNase MRP.

References cited on this page View Complete Literature Guide for PSO2

1)	Henriques JA and Moustacchi E (1980) Isolation and characterization of pso mutants sensitive to photo-addition of psoralen derivatives in Saccharomyces cerevisiae. Genetics 95(2):273-88
2)	Cassier-Chauvat C and Moustacchi E (1988) Allelism between pso1-1 and rev3-1 mutants and between pso2-1 and snm1 mutants in Saccharomyces cerevisiae. Curr Genet 13(1):37-40
3)	Cassier C and Moustacchi E (1981) Mutagenesis induced by mono- and bi-functional alkylating agents in yeast mutants sensitive to photo-addition of furocoumarins (pso). Mutat Res 84(1):37-47
4)	Wilborn F and Brendel M (1989) Formation and stability of interstrand cross-links induced by cis- and trans-diamminedichloroplatinum (II) in the DNA of Saccharomyces cerevisiae strains differing in repair capacity. Curr Genet 16(5-6):331-8
5)	Angulo JF, et al. (1986) Induction of polypeptides in Saccharomyces cerevisiae after ultraviolet irradiation. Biochem Biophys Res Commun 138(2):679-86
6)	Magana-Schwencke N, et al. (1982) The fate of 8-methoxypsoralen photoinduced crosslinks in nuclear and mitochondrial yeast DNA: comparison of wild-type and repair-deficient strains. Proc Natl Acad Sci U S A 79(6):1722-6
7)	Li X and Moses RE (2003) The beta-lactamase motif in Snm1 is required for repair of DNA double-strand breaks caused by interstrand crosslinks in S. cerevisiae. DNA Repair (Amst) 2(1):121-9
8)	Brendel M, et al. (2003) Role of PSO genes in repair of DNA damage of Saccharomyces cerevisiae. Mutat Res 544(2-3):179-93
9)	Tkach JM, et al. (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
10)	Ward TA, et al. (2012) Components of a fanconi-like pathway control pso2-independent DNA interstrand crosslink repair in yeast. PLoS Genet 8(8):e1002884