Standard Name	MMS22 1
Systematic Name	YLR320W
Alias	SLM2 2
Feature Type	ORF, Verified
Description	Subunit of an E3 ubiquitin ligase complex involved in replication repair; stabilizes protein components of the replication fork, such as the fork-pausing complex and leading strand polymerase, preventing fork collapse and promoting efficient recovery during replication stress; required for accurate meiotic chromosome segregation (1, 2, 3, 4, 5)
Name Description	Methyl MethaneSulfonate sensitivity 1

Chromosomal Location	ChrXII:771940 to 776304 | ORF Map | GBrowse

	View Computational GO annotations for MMS22
Molecular Function
Manually curated	molecular_function unknown (ND)
Biological Process
Manually curated	double-strand break repair (IMP) meiotic sister chromatid segregation (IMP) recombinational repair (IMP) replication fork processing (IMP) response to DNA damage stimulus (IMP)
Cellular Component
Manually curated	Cul8-RING ubiquitin ligase complex (IDA) nucleus (IDA)

Classical genetics
null	X ray resistance: decreased bud morphology: abnormal chromosome/plasmid maintenance: abnormal colony sectoring: increased heat sensitivity: increased meiosis: abnormal mitotic recombination: decreased mitotic recombination: normal oxidative stress resistance: decreased resistance to hydroxyurea: decreased resistance to methyl methanesulfonate: decreased resistance to camptothecin: decreased vegetative growth: decreased
overexpression	cell cycle progression through the G2/M phase transition: arrested
repressible	cell cycle progression through the G2/M phase transition: increased
Large-scale survey
null	auxotrophy bud morphology: abnormal cell size: increased glycogen accumulation: increased competitive fitness: decreased dessication resistance: decreased endocytosis: decreased fermentative growth: decreased rate heat sensitivity: increased resistance to bleomycin: increased resistance to caffeine: increased resistance to fluconazole: increased resistance to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO): decreased resistance to 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid: decreased resistance to methyl methanesulfonate: decreased resistance to tirapazamine: decreased resistance to formaldehyde: decreased resistance to camptothecin: decreased resistance to hydroxyurea: decreased resistance to sodium arsenite: decreased resistance to quinine: decreased resistance to 4-chlorophenol: decreased resistance to pentachlorophenol: decreased resistance to cycloheximide: decreased resistance to dimethyl sulfoxide: decreased resistance to caffeine: decreased resistance to doxorubicin: decreased toxin resistance: decreased transposable element transposition: increased vacuolar morphology: abnormal vegetative growth: decreased rate viable
Resources	PROPHECY | SCMD | ScreenTroll | Yeast Fitness Database

	469 total interaction(s) for 305 unique genes/features.
Physical Interactions	Affinity Capture-MS: 19 Affinity Capture-Western: 9 Two-hybrid: 7
Genetic Interactions	Dosage Lethality: 1 Negative Genetic: 213 Positive Genetic: 41 Synthetic Growth Defect: 136 Synthetic Lethality: 41 Synthetic Rescue: 2
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DIP | DRYGIN | GeneMANIA | InterologFinder | YeastRC Two-Hybrid

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP
Homologs	Ashbya (AGD) | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrXII:771940 to 776304 | ORF Map | GBrowse
Last Update	Coordinates: 2004-02-05 | Sequence: 1996-07-31
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..4365 771940..776304 2004-02-05 1996-07-31
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI1631 S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CEN.PK113-7D S.c. Strain EC1118 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain JAY291 S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain PW5 S.c. Strain RM11-1a S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain T73 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain YJM269 S.c. Strain YJM789 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000004312