ACF2/YLR144C Summary Help

Standard Name ACF2 1
Systematic Name YLR144C
Alias ENG2 , PCA1
Feature Type ORF, Verified
Description Intracellular beta-1,3-endoglucanase; expression is induced during sporulation; may have a role in cortical actin cytoskeleton assembly; protein abundance increases in response to DNA replication stress (1, 2, 3, 4)
Name Description Assembly Complementing Factor 1
Chromosomal Location
ChrXII:432016 to 429677 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All ACF2 GO evidence and references
  View Computational GO annotations for ACF2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 3 genes
Resources
Large-scale survey
null
Resources
42 total interaction(s) for 30 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 11
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 1
  • PCA: 10
  • Protein-peptide: 3
  • Two-hybrid: 12

Genetic Interactions
  • Negative Genetic: 1
  • Synthetic Growth Defect: 2

Resources
Expression Summary
histogram
Resources
Length (a.a.) 779
Molecular Weight (Da) 88,402
Isoelectric Point (pI) 5.15
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXII:432016 to 429677 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..2340 432016..429677 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004134
References cited on this page View Complete Literature Guide for ACF2
1) Lechler T and Li R  (1997) In vitro reconstitution of cortical actin assembly sites in budding yeast. J Cell Biol 138(1):95-103
2) Baladron V, et al.  (2002) Eng1p, an endo-1,3-beta-glucanase localized at the daughter side of the septum, is involved in cell separation in Saccharomyces cerevisiae. Eukaryot Cell 1(5):774-86
3) Martin-Cuadrado AB, et al.  (2008) Characterization of the endo-beta-1,3-glucanase activity of S. cerevisiae Eng2 and other members of the GH81 family. Fungal Genet Biol 45(4):542-53
4) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76