MRF1/YGL143C Summary Help

Standard Name MRF1 1
Systematic Name YGL143C
Feature Type ORF, Verified
Description Mitochondrial translation release factor; involved in stop codon recognition and hydrolysis of the peptidyl-tRNA bond during mitochondrial translation; lack of MRF1 causes mitochondrial genome instability (1, 2 and see Summary Paragraph)
Name Description Mitochondrial peptide chain Release Factor 1
Chromosomal Location
ChrVII:235958 to 234717 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All MRF1 GO evidence and references
  View Computational GO annotations for MRF1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 4 genes
Resources
Classical genetics
null
Large-scale survey
null
repressible
Resources
14 total interaction(s) for 12 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 1
  • Affinity Capture-RNA: 1
  • Biochemical Activity: 2
  • Two-hybrid: 2

Genetic Interactions
  • Synthetic Growth Defect: 6
  • Synthetic Rescue: 2

Resources
Expression Summary
histogram
Resources
Length (a.a.) 413
Molecular Weight (Da) 46,770
Isoelectric Point (pI) 9.51
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrVII:235958 to 234717 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1242 235958..234717 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003111
SUMMARY PARAGRAPH for MRF1

MRF1 encodes a mitochondrial peptide chain release factor that terminates translation in response to the termination codons UAA and UAG (1, 3). MRF1 has homologs in E. coli and Kluyveromyces lactis (1, 4). mrf1 mutants exhibit respiratory growth defects, and expression of the Kluyveromyces lactis MRF1 gene complements this phenotype (1, 4).

Note: ETR1/YBR026C encoding a 2-enoyl thioester reductase has also been referred to as MRF1 in the literature.

Last updated: 2000-06-09 Contact SGD

References cited on this page View Complete Literature Guide for MRF1
1) Pel HJ, et al.  (1992) The yeast nuclear gene MRF1 encodes a mitochondrial peptide chain release factor and cures several mitochondrial RNA splicing defects. Nucleic Acids Res 20(23):6339-46
2) Towpik J, et al.  (2004) Mutations in the yeast mrf1 gene encoding mitochondrial release factor inhibit translation on mitochondrial ribosomes. J Biol Chem 279(14):14096-103
3) Askarian-Amiri ME, et al.  (2000) Functional characterization of yeast mitochondrial release factor 1. J Biol Chem 275(23):17241-8
4) Pel HJ, et al.  (1996) The nuclear Kluyveromyces lactis MRF1 gene encodes a mitochondrial class I peptide chain release factor that is important for cell viability. Curr Genet 30(1):19-28