CDH1/YGL003C Summary Help

Standard Name CDH1 1
Systematic Name YGL003C
Alias HCT1 2
Feature Type ORF, Verified
Description Cell-cycle regulated activator of the anaphase-promoting complex/cyclosome (APC/C), which directs ubiquitination of cyclins resulting in mitotic exit; targets the APC/C to specific substrates including Cdc20p, Ase1p, Cin8p and Fin1p (1, 3, 4 and see Summary Paragraph)
Name Description CDC20 Homolog 1
Chromosomal Location
ChrVII:494174 to 492474 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All CDH1 GO evidence and references
  View Computational GO annotations for CDH1
Molecular Function
Manually curated
Biological Process
Manually curated
High-throughput
Cellular Component
Manually curated
Mutant phenotypes All CDH1 Phenotype evidence and references
Classical genetics
null
overexpression
reduction of function
unspecified
Large-scale survey
null
overexpression
Resources
interactions All CDH1 Interaction evidence and references
416 total interaction(s) for 268 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 66
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 45
  • Biochemical Activity: 23
  • Co-crystal Structure: 2
  • Protein-peptide: 1
  • Reconstituted Complex: 8
  • Two-hybrid: 12
Genetic Interactions
  • Dosage Growth Defect: 8
  • Dosage Lethality: 6
  • Dosage Rescue: 8
  • Negative Genetic: 101
  • Phenotypic Enhancement: 16
  • Phenotypic Suppression: 12
  • Positive Genetic: 32
  • Synthetic Growth Defect: 48
  • Synthetic Lethality: 24
  • Synthetic Rescue: 3
Resources
Expression Summary
histogram
Resources
Protein Information All CDH1 Protein evidence and references
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrVII:494174 to 492474 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates		 Chromosomal
Coordinates		 Most Recent Updates
Coordinates Sequence
CDS 1..1701 494174..492474 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002971
SUMMARY PARAGRAPH for CDH1

CDH1 is an activator of the anaphase-promoting complex (APC), an E3 ubiquitin ligase in the ubiquitin-mediated proteolysis pathway (5). The APC ubiquitin ligase helps regulate the metaphase/anaphase transition and exit from mitosis/G1 entry through ubiquitination of various substrates. These include mitotic cyclins, the sister chromatid separation inhibitor Pds1p, the Kip1p and Cin8p motor proteins, Cdc5p, and the spindle disassemblyfactor, Ase1p (6, 7, 8, 9, 10, 11).

Deletion of Cdh1 results in slow growth and the accumulation of very large amounts of Clb2p and Ase1p (1). Further analysis of the mutants demonstrated that Cdh1p regulates the activity and substrate specificity of the APC (1). It serves as an activator of the APC and mediates ubiquitin-dependent protein degradation of the mitotic cyclin Clb2p, the polo kinase Cdc5p, and the spindle protein Ase1p during anaphase of the cell cycle (2, 1, 12).

The level of Cdh1p remains constant throughout the cell cycle, however it only binds the APC during G1. For the rest of the cell cycle, the association of Cdh1p with the APC is inhibited due to its phosphorylation by the kinase Cdc28p (13, 14).

CDH1 is a homolog of CDC20, another APC activator (1). CDH1 orthologs have been identified in various species including worms, flies and humans (15, 5, 1).

Last updated: 2005-05-11

References cited on this page View Complete Literature Guide for CDH1
1) Visintin R, et al.  (1997) CDC20 and CDH1: a family of substrate-specific activators of APC-dependent proteolysis. Science 278(5337):460-3
2) Schwab M, et al.  (1997) Yeast Hct1 is a regulator of Clb2 cyclin proteolysis. Cell 90(4):683-93
3) Harper JW, et al.  (2002) The anaphase-promoting complex: it's not just for mitosis any more. Genes Dev 16(17):2179-206
4) Woodbury EL and Morgan DO  (2007) Cdk and APC activities limit the spindle-stabilizing function of Fin1 to anaphase. Nat Cell Biol 9(1):106-12
5) Zachariae W and Nasmyth K  (1999) Whose end is destruction: cell division and the anaphase-promoting complex. Genes Dev 13(16):2039-58
6) Juang YL, et al.  (1997) APC-mediated proteolysis of Ase1 and the morphogenesis of the mitotic spindle. Science 275(5304):1311-4
7) Cohen-Fix O, et al.  (1996) Anaphase initiation in Saccharomyces cerevisiae is controlled by the APC-dependent degradation of the anaphase inhibitor Pds1p. Genes Dev 10(24):3081-93
8) Shirayama M, et al.  (1998) The Polo-like kinase Cdc5p and the WD-repeat protein Cdc20p/fizzy are regulators and substrates of the anaphase promoting complex in Saccharomyces cerevisiae. EMBO J 17(5):1336-49
9) Gordon DM and Roof DM  (2001) Degradation of the kinesin Kip1p at anaphase onset is mediated by the anaphase-promoting complex and Cdc20p. Proc Natl Acad Sci U S A 98(22):12515-20
10) Hildebrandt ER and Hoyt MA  (2001) Cell cycle-dependent degradation of the Saccharomyces cerevisiae spindle motor Cin8p requires APC(Cdh1) and a bipartite destruction sequence. Mol Biol Cell 12(11):3402-16
11) Zachariae W and Nasmyth K  (1996) TPR proteins required for anaphase progression mediate ubiquitination of mitotic B-type cyclins in yeast. Mol Biol Cell 7(5):791-801
12) Charles JF, et al.  (1998) The Polo-related kinase Cdc5 activates and is destroyed by the mitotic cyclin destruction machinery in S. cerevisiae. Curr Biol 8(9):497-507
13) Zachariae W, et al.  (1998) Control of cyclin ubiquitination by CDK-regulated binding of Hct1 to the anaphase promoting complex. Science 282(5394):1721-4
14) Prinz S, et al.  (1998) The regulation of Cdc20 proteolysis reveals a role for APC components Cdc23 and Cdc27 during S phase and early mitosis. Curr Biol 8(13):750-60
15) Dawson IA, et al.  (1995) The Drosophila cell cycle gene fizzy is required for normal degradation of cyclins A and B during mitosis and has homology to the CDC20 gene of Saccharomyces cerevisiae. J Cell Biol 129(3):725-37