PAP1/YKR002W Summary Help

Standard Name PAP1 1
Systematic Name YKR002W
Feature Type ORF, Verified
Description Poly(A) polymerase; one of three factors required for mRNA 3'-end polyadenylation, forms multiprotein complex with polyadenylation factor I (PF I), also required for mRNA nuclear export; may also polyadenylate rRNAs; required for gene looping (2, 3, 4, 5, 6)
Name Description Poly(A) Polymerase 1
Chromosomal Location
ChrXI:443232 to 444938 | ORF Map | GBrowse
Gbrowse
Genetic position: -1 cM
Gene Ontology Annotations All PAP1 GO evidence and references
  View Computational GO annotations for PAP1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 5 genes
Resources
Classical genetics
conditional
Large-scale survey
null
overexpression
reduction of function
repressible
Resources
157 total interaction(s) for 48 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 101
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 16
  • Biochemical Activity: 2
  • Co-crystal Structure: 1
  • Co-localization: 1
  • Co-purification: 1
  • Reconstituted Complex: 8
  • Two-hybrid: 9

Genetic Interactions
  • Dosage Growth Defect: 1
  • Dosage Lethality: 1
  • Phenotypic Enhancement: 2
  • Phenotypic Suppression: 2
  • Positive Genetic: 2
  • Synthetic Growth Defect: 3
  • Synthetic Haploinsufficiency: 1
  • Synthetic Lethality: 3
  • Synthetic Rescue: 2

Resources
Expression Summary
histogram
Resources
Length (a.a.) 568
Molecular Weight (Da) 64,551
Isoelectric Point (pI) 7.87
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXI:443232 to 444938 | ORF Map | GBrowse
SGD ORF map
Genetic position: -1 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1707 443232..444938 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001710
References cited on this page View Complete Literature Guide for PAP1
1) Lingner J, et al.  (1991) Cloning and expression of the essential gene for poly(A) polymerase from S. cerevisiae. Nature 354(6353):496-8
2) Preker PJ, et al.  (1997) A multisubunit 3' end processing factor from yeast containing poly(A) polymerase and homologues of the subunits of mammalian cleavage and polyadenylation specificity factor. EMBO J 16(15):4727-37
3) Mizrahi N and Moore C  (2000) Posttranslational phosphorylation and ubiquitination of the Saccharomyces cerevisiae Poly(A) polymerase at the S/G(2) stage of the cell cycle. Mol Cell Biol 20(8):2794-802
4) Brodsky AS and Silver PA  (2000) Pre-mRNA processing factors are required for nuclear export. RNA 6(12):1737-49
5) Kuai L, et al.  (2004) Polyadenylation of rRNA in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A 101(23):8581-6
6) Medler S, et al.  (2011) Evidence for a complex of transcription factor IIB with poly(A) polymerase and cleavage factor 1 subunits required for gene looping. J Biol Chem 286(39):33709-18