MIF2/YKL089W Summary Help

Standard Name MIF2 1, 2
Systematic Name YKL089W
Feature Type ORF, Verified
Description Protein required for structural integrity of elongating spindles; localizes to the kinetochore; interacts with histones H2A, H2B, and H4; phosphorylated by Ipl1p; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-C and fission yeast cnp3 (1, 3, 4, 5)
Name Description MItotic Fidelity of chromosome transmission 2
Chromosomal Location
ChrXI:273394 to 275043 | ORF Map | GBrowse
Gbrowse
Genetic position: -43 cM
Gene Ontology Annotations All MIF2 GO evidence and references
  View Computational GO annotations for MIF2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 3 genes
Resources
Classical genetics
conditional
null
overexpression
unspecified
Large-scale survey
conditional
null
Resources
87 total interaction(s) for 60 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 21
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 5
  • Biochemical Activity: 5
  • Co-crystal Structure: 2
  • Co-localization: 1
  • Reconstituted Complex: 5
  • Two-hybrid: 14

Genetic Interactions
  • Dosage Rescue: 1
  • Negative Genetic: 1
  • Phenotypic Enhancement: 4
  • Positive Genetic: 1
  • Synthetic Growth Defect: 23
  • Synthetic Lethality: 3

Resources
Expression Summary
histogram
Resources
Length (a.a.) 549
Molecular Weight (Da) 62,472
Isoelectric Point (pI) 4.49
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXI:273394 to 275043 | ORF Map | GBrowse
SGD ORF map
Genetic position: -43 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1650 273394..275043 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001572
References cited on this page View Complete Literature Guide for MIF2
1) Brown MT, et al.  (1993) MIF2 is required for mitotic spindle integrity during anaphase spindle elongation in Saccharomyces cerevisiae. J Cell Biol 123(2):387-403
2) Meeks-Wagner D, et al.  (1986) Isolation of two genes that affect mitotic chromosome transmission in S. cerevisiae. Cell 44(1):53-63
3) Meluh PB and Koshland D  (1995) Evidence that the MIF2 gene of Saccharomyces cerevisiae encodes a centromere protein with homology to the mammalian centromere protein CENP-C. Mol Biol Cell 6(7):793-807
4) Westermann S, et al.  (2003) Architecture of the budding yeast kinetochore reveals a conserved molecular core. J Cell Biol 163(2):215-22
5) Schleiffer A, et al.  (2012) CENP-T proteins are conserved centromere receptors of the Ndc80 complex. Nat Cell Biol 14(6):604-13