IMP2'/YIL154C Summary

Standard Name	IMP2' 1 (see Nomenclature conflict Note)
Systematic Name	YIL154C
Alias	IMP2 2
Feature Type	ORF, Verified
Description	Transcriptional activator involved in maintenance of ion homeostasis and protection against DNA damage caused by bleomycin and other oxidants, contains a C-terminal leucine-rich repeat (3, 4 and see Summary Paragraph)
Name Description	Independent of Mitochondria1 Particle 2

Chromosomal Location	ChrIX:55021 to 53981 \| ORF Map \| GBrowse
	Note: this feature is encoded on the Crick strand.

Gene Ontology Annotations All IMP2' GO evidence and references


Molecular Function
Manually curated	transcription coactivator activity (IDA)
Biological Process
Manually curated	cellular carbohydrate metabolic process (IMP) DNA repair (IMP)
Cellular Component
High-throughput	cytoplasm (IDA)

Mutant phenotypes All IMP2' Phenotype evidence and references

Classical genetics
null	lithium(1+) accumulation: decreased gamma ray resistance: decreased resistance to tert-butylhydroperoxide: decreased resistance to bleomycin: decreased utilization of carbon source: decreased viable
unspecified	killer toxin resistance: increased resistance to caffeine: decreased resistance to hygromycin B: decreased resistance to enzymatic treatment: increased
Large-scale survey
null	bud morphology: abnormal cell size: increased competitive fitness: decreased dessication resistance: decreased endocytosis: decreased haploproficient hyperosmotic stress resistance: decreased ionic stress resistance: decreased metal resistance: decreased metal resistance: increased oxidative stress resistance: decreased resistance to chloroquine: decreased resistance to amitrole: decreased resistance to streptomycin: decreased resistance to L-1,4-dithiothreitol: decreased resistance to cycloheximide: decreased resistance to hygromycin B: decreased resistance to calcium dichloride: decreased resistance to cisplatin: decreased resistance to doxorubicin: decreased respiratory growth: decreased rate respiratory growth: absent sporulation: absent toxin resistance: decreased vegetative growth: abnormal viable
unspecified	resistance to Calcofluor White: decreased
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All IMP2' Interaction evidence and references

	76 total interaction(s) for 71 unique genes/features.
Physical Interactions	Affinity Capture-MS: 12 Biochemical Activity: 1
Genetic Interactions	Dosage Growth Defect: 2 Dosage Rescue: 3 Negative Genetic: 40 Phenotypic Enhancement: 1 Phenotypic Suppression: 1 Positive Genetic: 1 Synthetic Growth Defect: 6 Synthetic Haploinsufficiency: 1 Synthetic Lethality: 4 Synthetic Rescue: 4
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All IMP2' Protein evidence and references

Localization	YeastRC \| YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrIX:55021 to 53981 | |

Note: this feature is encoded on the Crick strand.

Last Update

Coordinates: 1994-12-10 | Sequence: 1994-12-10

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..1041	55021..53981	1994-12-10	1994-12-10

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000001416

NOMENCLATURE CONFLICT NOTE

Name	Relevance	Description
INM2	Nomenclature conflict	The gene name IMP2 has been used to refer to the transcriptional coactivator IMP2'/YIL154C, the inositol monophosphatase INM2/YDR287W, and the mitochondrial inner membrane peptidase IMP2/YMR035W.
IMP2	Nomenclature conflict	The gene name IMP2 has been used to refer to the transcriptional coactivator IMP2'/YIL154C, the inositol monophosphatase INM2/YDR287W, and the mitochondrial inner membrane peptidase IMP2/YMR035W.

SUMMARY PARAGRAPH for IMP2'

IMP2' was originally isolated as a mutant defective in maltose, galactose and raffinose metabolism (2). IMP2' is required for the rapid glucose derepression of the maltose, galactose, raffinose and ethanol utilization pathways, and it has been shown that Imp2'p regulates galactose- and maltose-inducible genes at the transcriptional level (5). In addition, molecular analysis of IMP2' indicates that it encodes a transcription factor that can activate a reporter gene (4). IMP2' was also isolated in a screen for bleomycin mutants; bleomycin is an antitumor drug that damages DNA by producing free radicals (4). imp2' mutants are sensitive to other DNA damaging agents, suggesting that Imp2p' is involved in regulation of genes involved in DNA repair (4).

Note: this gene is designated IMP2' in SGD because another gene has also been referred to as IMP2 in the literature. The other IMP2 gene (ORF YMR035W) is a mitochondrial protease that cleaves signal sequences from proteins targeted to the mitochondrial intermembrane space.

Last updated: 2000-06-09

References cited on this page View Complete Literature Guide for IMP2'

1)	Donnini, C. (1992) Personal Communication, Mortimer Map Edition 11
2)	Donnini C, et al. (1992) IMP2, a nuclear gene controlling the mitochondrial dependence of galactose, maltose and raffinose utilization in Saccharomyces cerevisiae. Yeast 8(2):83-93
3)	Masson JY and Ramotar D (1998) The transcriptional activator Imp2p maintains ion homeostasis in Saccharomyces cerevisiae. Genetics 149(2):893-901
4)	Masson JY and Ramotar D (1996) The Saccharomyces cerevisiae IMP2 gene encodes a transcriptional activator that mediates protection against DNA damage caused by bleomycin and other oxidants. Mol Cell Biol 16(5):2091-100
5)	Lodi T, et al. (1995) IMP2, a gene involved in the expression of glucose-repressible genes in Saccharomyces cerevisiae. Microbiology 141 ( Pt 9):2201-9