SLT2/YHR030C Summary Help

Standard Name SLT2 1
Systematic Name YHR030C
Alias BYC2 , MPK1 2 , SLK2 , LYT2 3
Feature Type ORF, Verified
Description Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, cell cycle progression, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to phagophore assembly site (PAS); plays a role in adaptive response of cells to cold; regulated by the PKC1-mediated signaling pathway; SLT2 has a paralog, KDX1, that arose from the whole genome duplication (4, 5, 6, 7, 8, 9, 10, 11 and see Summary Paragraph)
Name Description Suppressor of the LyTic phenotype 1
Chromosomal Location
ChrVIII:170344 to 168890 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All SLT2 GO evidence and references
  View Computational GO annotations for SLT2
Molecular Function
Manually curated
High-throughput
Biological Process
Manually curated
High-throughput
Cellular Component
Manually curated
High-throughput
Regulators 3 genes
Resources
Classical genetics
null
Large-scale survey
null
repressible
Resources
1178 total interaction(s) for 619 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 60
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 27
  • Biochemical Activity: 26
  • Co-fractionation: 2
  • Co-localization: 4
  • Co-purification: 1
  • PCA: 10
  • Reconstituted Complex: 14
  • Two-hybrid: 20

Genetic Interactions
  • Dosage Growth Defect: 192
  • Dosage Lethality: 8
  • Dosage Rescue: 48
  • Negative Genetic: 456
  • Phenotypic Enhancement: 19
  • Phenotypic Suppression: 19
  • Positive Genetic: 40
  • Synthetic Growth Defect: 77
  • Synthetic Lethality: 137
  • Synthetic Rescue: 16

Resources
Expression Summary
histogram
Resources
Length (a.a.) 484
Molecular Weight (Da) 55,636
Isoelectric Point (pI) 5.07
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrVIII:170344 to 168890 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1455 170344..168890 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001072
SUMMARY PARAGRAPH for SLT2

The Pkc1p kinase controls a highly-conserved cell wall integrity signalling pathway that regulates functions essential for growth and the integrity of proliferating cells (2, 12, 3). This pathway consists of a cascade of phosphorylation reactions initiated with the activation of Pkc1p (13, 14). Pkc1p then activates a basic three-protein kinase module involving an integration of the MEK-kinase Bck1p (15, 16), the redundant MEK-kinases Mkk1p and Mkk2p (17), and the MAP kinase Slt2p (1, 2). Strains disrupted for any of these protein kinases lose osmotic stability, especially at 37 C, such that the cells are viable only in the presence of sorbitol (or some other osmotic stabilizer), suggesting that the lysis is due to lack of cell wall integrity (3, 18).

Sdp1p specifically interacts with the stress-activated Slt2p MAP kinase, and expression of an inactive mutant of Sdp1p causes the accumulation of phosphorylated Slt2p, indicating that Slt2p is a physiological target of the Sdp1p phosphatase (19). Heat shock-induced Slt2p phosphorylation levels are elevated in the absence of Sdp1p (20) and Sdp1p is required for the dephosphorylation of Slt2p during oxidative conditions (21).

Last updated: 2005-03-22 Contact SGD

References cited on this page View Complete Literature Guide for SLT2
1) Torres L, et al.  (1991) A protein kinase gene complements the lytic phenotype of Saccharomyces cerevisiae lyt2 mutants. Mol Microbiol 5(11):2845-54
2) Lee KS, et al.  (1993) A yeast mitogen-activated protein kinase homolog (Mpk1p) mediates signalling by protein kinase C. Mol Cell Biol 13(5):3067-75
3) Martin H, et al.  (1996) Molecular and functional characterization of a mutant allele of the mitogen-activated protein-kinase gene SLT2(MPK1) rescued from yeast autolytic mutants. Curr Genet 29(6):516-22
4) Watanabe Y, et al.  (1995) Yeast RLM1 encodes a serum response factor-like protein that may function downstream of the Mpk1 (Slt2) mitogen-activated protein kinase pathway. Mol Cell Biol 15(10):5740-9
5) Madden K, et al.  (1997) SBF cell cycle regulator as a target of the yeast PKC-MAP kinase pathway. Science 275(5307):1781-4
6) Martin-Yken H, et al.  (2003) The interaction of Slt2 MAP kinase with Knr4 is necessary for signalling through the cell wall integrity pathway in Saccharomyces cerevisiae. Mol Microbiol 49(1):23-35
7) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
8) Kim KY, et al.  (2010) Yeast Mpk1 cell wall integrity mitogen-activated protein kinase regulates nucleocytoplasmic shuttling of the Swi6 transcriptional regulator. Mol Biol Cell 21(9):1609-19
9) Carmody SR, et al.  (2010) The Mitogen-Activated Protein Kinase Slt2 Regulates Nuclear Retention of Non-Heat Shock mRNAs during Heat Shock-Induced Stress. Mol Cell Biol 30(21):5168-79
10) Mao K, et al.  (2011) Two MAPK-signaling pathways are required for mitophagy in Saccharomyces cerevisiae. J Cell Biol 193(4):755-67
11) Corcoles-Saez I, et al.  (2012) Low temperature highlights the functional role of the cell wall integrity pathway in the regulation of growth in Saccharomyces cerevisiae. Biochem J 446(3):477-88
12) Sussman A, et al.  (2004) Discovery of cercosporamide, a known antifungal natural product, as a selective Pkc1 kinase inhibitor through high-throughput screening. Eukaryot Cell 3(4):932-43
13) Levin DE, et al.  (1990) A candidate protein kinase C gene, PKC1, is required for the S. cerevisiae cell cycle. Cell 62(2):213-24
14) Paravicini G, et al.  (1992) The osmotic integrity of the yeast cell requires a functional PKC1 gene product. Mol Cell Biol 12(11):4896-905
15) Costigan C, et al.  (1994) NHP6A and NHP6B, which encode HMG1-like proteins, are candidates for downstream components of the yeast SLT2 mitogen-activated protein kinase pathway. Mol Cell Biol 14(4):2391-403
16) Lee KS and Levin DE  (1992) Dominant mutations in a gene encoding a putative protein kinase (BCK1) bypass the requirement for a Saccharomyces cerevisiae protein kinase C homolog. Mol Cell Biol 12(1):172-82
17) Irie K, et al.  (1993) MKK1 and MKK2, which encode Saccharomyces cerevisiae mitogen-activated protein kinase-kinase homologs, function in the pathway mediated by protein kinase C. Mol Cell Biol 13(5):3076-83
18) Cid VJ, et al.  (1995) Molecular basis of cell integrity and morphogenesis in Saccharomyces cerevisiae. Microbiol Rev 59(3):345-86
19) Collister M, et al.  (2002) YIL113w encodes a functional dual-specificity protein phosphatase which specifically interacts with and inactivates the Slt2/Mpk1p MAP kinase in S. cerevisiae. FEBS Lett 527(1-3):186-92
20) Hahn JS and Thiele DJ  (2002) Regulation of the Saccharomyces cerevisiae Slt2 kinase pathway by the stress-inducible Sdp1 dual specificity phosphatase. J Biol Chem 277(24):21278-84
21) Fox GC, et al.  (2007) Redox-mediated substrate recognition by Sdp1 defines a new group of tyrosine phosphatases. Nature 447(7143):487-92