MRC1/YCL061C Summary Help

Standard Name MRC1 1
Systematic Name YCL061C
Alias YCL060C
Feature Type ORF, Verified
Description S-phase checkpoint protein required for DNA replication; couples DNA helicase and DNA polymerase; interacts with and stabilizes Pol2p at stalled replication forks during stress, where it forms a pausing complex with Tof1p and is phosphorylated by Mec1p; with Hog1p defines a novel S-phase checkpoint that permits eukaryotic cells to prevent conflicts between DNA replication and transcription; protects uncapped telomeres; degradation via Dia2p help cells resume cell cycle (2, 3, 4, 5, 6, 7)
Name Description Mediator of the Replication Checkpoint 1
Chromosomal Location
ChrIII:22106 to 18816 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All MRC1 GO evidence and references
  View Computational GO annotations for MRC1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 4 genes
Resources
Classical genetics
null
reduction of function
Large-scale survey
null
overexpression
Resources
705 total interaction(s) for 284 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 17
  • Affinity Capture-Western: 36
  • Biochemical Activity: 4
  • Reconstituted Complex: 1
  • Two-hybrid: 9

Genetic Interactions
  • Dosage Growth Defect: 2
  • Dosage Lethality: 2
  • Dosage Rescue: 9
  • Negative Genetic: 278
  • Phenotypic Enhancement: 42
  • Phenotypic Suppression: 9
  • Positive Genetic: 37
  • Synthetic Growth Defect: 147
  • Synthetic Lethality: 97
  • Synthetic Rescue: 15

Resources
Expression Summary
histogram
Resources
Length (a.a.) 1,096
Molecular Weight (Da) 124,325
Isoelectric Point (pI) 5.03
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrIII:22106 to 18816 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2000-09-13 | Sequence: 2000-09-13
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..3291 22106..18816 2000-09-13 2000-09-13
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000566
References cited on this page View Complete Literature Guide for MRC1
1) Alcasabas AA, et al.  (2001) Mrc1 transduces signals of DNA replication stress to activate Rad53. Nat Cell Biol 3(11):958-65
2) Osborn AJ and Elledge SJ  (2003) Mrc1 is a replication fork component whose phosphorylation in response to DNA replication stress activates Rad53. Genes Dev 17(14):1755-67
3) Katou Y, et al.  (2003) S-phase checkpoint proteins Tof1 and Mrc1 form a stable replication-pausing complex. Nature 424(6952):1078-83
4) Tsolou A and Lydall D  (2007) Mrc1 protects uncapped budding yeast telomeres from exonuclease EXO1. DNA Repair (Amst) 6(11):1607-1617
5) Lou H, et al.  (2008) Mrc1 and DNA polymerase epsilon function together in linking DNA replication and the S phase checkpoint. Mol Cell 32(1):106-17
6) Fong CM, et al.  (2013) The Saccharomyces cerevisiae F-Box Protein Dia2 Is a Mediator of S-Phase Checkpoint Recovery from DNA Damage. Genetics 193(2):483-99
7) Duch A, et al.  (2013) Coordinated control of replication and transcription by a SAPK protects genomic integrity. Nature 493(7430):116-9