PCA1/YBR295W Summary Help

Standard Name PCA1 1
Systematic Name YBR295W
Alias CAD2 2 , PAY2
Feature Type ORF, Verified
Description Cadmium transporting P-type ATPase; may also have a role in copper and iron homeostasis; stabilized by Cd binding, which prevents ubiquitination; S288C and other lab strains contain a G970R mutation which eliminates Cd transport function (1, 2, 3, 4, 5 and see Summary Paragraph)
Name Description P-type Cation-transporting ATPase 1
Chromosomal Location
ChrII:792849 to 796499 | ORF Map | GBrowse
Genetic position: 161 cM
Gene Ontology Annotations All PCA1 GO evidence and references
  View Computational GO annotations for PCA1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 2 genes
Classical genetics
gain of function
Large-scale survey
28 total interaction(s) for 22 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 8
  • Affinity Capture-Western: 2
  • PCA: 1

Genetic Interactions
  • Negative Genetic: 11
  • Positive Genetic: 5
  • Synthetic Lethality: 1

Expression Summary
Length (a.a.) 1,216
Molecular Weight (Da) 131,875
Isoelectric Point (pI) 7.55
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrII:792849 to 796499 | ORF Map | GBrowse
Genetic position: 161 cM
Last Update Coordinates: 2011-02-03 | Sequence: 2011-02-03
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..3651 792849..796499 2011-02-03 2011-02-03
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000499

PCA1 encodes a plasma membrane P-type ATPase ion pump responsible for the export of toxic cadmium ions. Early studies of Pca1p were complicated by the fact that most laboratory strains, including S288C, contain a G970R nonsense mutation which eliminates normal cadmium transport function (2, 4). Although initial characterizations described Pca1p as a copper-transporting ATPase (1), subsequent experiments have demonstrated that Pca1p is capable of high affinity copper ion binding, but not active copper ion transport. It is possible that Pca1p plays a role in yeast copper resistance by chelating and sequestering copper ions (4). Pca1p is very closely related to the human gene ATP7A, mutations in which cause Menkes disease ('kinky hair' disease) and occipital horn syndrome. Menkes disease is associated with defective copper metabolism (6).

Last updated: 2007-02-23 Contact SGD

References cited on this page View Complete Literature Guide for PCA1
1) Rad MR, et al.  (1994) A putative P-type Cu(2+)-transporting ATPase gene on chromosome II of Saccharomyces cerevisiae. Yeast 10(9):1217-25
2) Shiraishi E, et al.  (2000) The cadmium-resistant gene, CAD2, which is a mutated putative copper-transporter gene (PCA1), controls the intracellular cadmium-level in the yeast S. cerevisiae. Curr Genet 37(2):79-86
3) De Freitas JM, et al.  (2004) Exploratory and confirmatory gene expression profiling of mac1Delta. J Biol Chem 279(6):4450-8
4) Adle DJ, et al.  (2007) A cadmium-transporting P1B-type ATPase in yeast Saccharomyces cerevisiae. J Biol Chem 282(2):947-55
5) Adle DJ and Lee J  (2008) Expressional Control of a Cadmium-transporting P1B-type ATPase by a Metal Sensing Degradation Signal. J Biol Chem 283(46):31460-8
6) Pena MM, et al.  (1999) A delicate balance: homeostatic control of copper uptake and distribution. J Nutr 129(7):1251-60