PCA1/YBR295W Summary

Standard Name	PCA1 1 (see Nomenclature conflict Note)
Systematic Name	YBR295W
Alias	CAD2 2 , PAY2
Feature Type	ORF, Verified
Description	Cadmium transporting P-type ATPase; may also have a role in copper and iron homeostasis; stabilized by Cd binding, which prevents ubiquitination; S288C and other lab strains contain a G970R mutation which eliminates Cd transport function (1, 2, 3, 4, 5 and see Summary Paragraph)
Name Description	P-type Cation-transporting ATPase 1

Chromosomal Location	ChrII:792849 to 796499 \| ORF Map \| GBrowse

	Genetic position: 161 cM

Gene Ontology Annotations All PCA1 GO evidence and references

	View Computational GO annotations for PCA1
Molecular Function
Manually curated	ATPase activity, coupled to transmembrane movement of ions, phosphorylative mechanism (ISS) cadmium-exporting ATPase activity (IMP) copper ion binding (IMP) NOT copper-transporting ATPase activity (IMP)
Biological Process
Manually curated	cadmium ion transport (IGI, IMP) cellular iron ion homeostasis (IEP) cellular metal ion homeostasis (IMP) transmembrane transport (IMP)
Cellular Component
Manually curated	plasma membrane (IDA)
High-throughput	integral to membrane (ISM)

Mutant phenotypes All PCA1 Phenotype evidence and references

Classical genetics
gain of function	metal resistance: increased
null	metal resistance: decreased viable
overexpression	vegetative growth: decreased rate
Large-scale survey
null	competitive fitness: decreased haploinsufficient heat sensitivity: increased resistance to sodium selenide: decreased resistance to arsenite(3-): decreased toxin resistance: decreased vacuolar morphology: abnormal viable
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All PCA1 Interaction evidence and references

	26 total interaction(s) for 23 unique genes/features.
Physical Interactions	Affinity Capture-MS: 5 Affinity Capture-Western: 2 PCA: 1
Genetic Interactions	Negative Genetic: 12 Positive Genetic: 5 Synthetic Lethality: 1
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All PCA1 Protein evidence and references

Localization	YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrII:792849 to 796499 | |

: 161 cM

Last Update

Coordinates: 2011-02-03 | Sequence: 2011-02-03

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..3651	792849..796499	2011-02-03	2011-02-03

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| E.C. \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000000499

NOMENCLATURE CONFLICT NOTE

Name	Relevance	Description
ACF2	Nomenclature conflict	Both PCA1/YBR295W, which encodes a cation-transporting ATPase, and ACF2/YLR144C, which encodes a glucan 1,3-beta-glucosidase, have been referred to as PCA1.

SUMMARY PARAGRAPH for PCA1

PCA1 encodes a plasma membrane P-type ATPase ion pump responsible for the export of toxic cadmium ions. Early studies of Pca1p were complicated by the fact that most laboratory strains, including S288C, contain a G970R nonsense mutation which eliminates normal cadmium transport function (2, 4). Although initial characterizations described Pca1p as a copper-transporting ATPase (1), subsequent experiments have demonstrated that Pca1p is capable of high affinity copper ion binding, but not active copper ion transport. It is possible that Pca1p plays a role in yeast copper resistance by chelating and sequestering copper ions (4). Pca1p is very closely related to the human gene ATP7A, mutations in which cause Menkes disease ('kinky hair' disease) and occipital horn syndrome. Menkes disease is associated with defective copper metabolism (6).

Last updated: 2007-02-23

References cited on this page View Complete Literature Guide for PCA1

1)	Rad MR, et al. (1994) A putative P-type Cu(2+)-transporting ATPase gene on chromosome II of Saccharomyces cerevisiae. Yeast 10(9):1217-25
2)	Shiraishi E, et al. (2000) The cadmium-resistant gene, CAD2, which is a mutated putative copper-transporter gene (PCA1), controls the intracellular cadmium-level in the yeast S. cerevisiae. Curr Genet 37(2):79-86
3)	De Freitas JM, et al. (2004) Exploratory and confirmatory gene expression profiling of mac1Delta. J Biol Chem 279(6):4450-8
4)	Adle DJ, et al. (2007) A cadmium-transporting P1B-type ATPase in yeast Saccharomyces cerevisiae. J Biol Chem 282(2):947-55
5)	Adle DJ and Lee J (2008) Expressional Control of a Cadmium-transporting P1B-type ATPase by a Metal Sensing Degradation Signal. J Biol Chem 283(46):31460-8
6)	Pena MM, et al. (1999) A delicate balance: homeostatic control of copper uptake and distribution. J Nutr 129(7):1251-60