PFF1/YBR074W Summary Help

Standard Name PFF1 1
Systematic Name YBR074W
Alias YBR075W
Feature Type ORF, Verified
Description Multi-spanning vacuolar membrane protease; glycosylated transmembrane protein bearing homology to the M28 family of metalloproteases; has a lumenal-facing protease domain; proposed role in vacuole physiology (1, 2)
Name Description Protease in FXNA-related Family 1
Chromosomal Location
ChrII:386286 to 389216 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All PFF1 GO evidence and references
  View Computational GO annotations for PFF1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 3 genes
Resources
Classical genetics
null
Large-scale survey
null
Resources
9 total interaction(s) for 8 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 2
  • Co-fractionation: 1
  • Reconstituted Complex: 1

Genetic Interactions
  • Negative Genetic: 4
  • Positive Genetic: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 976
Molecular Weight (Da) 111,035
Isoelectric Point (pI) 6.51
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrII:386286 to 389216 | ORF Map | GBrowse
This feature contains embedded feature(s): YBR075W
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 2011-02-03
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..2931 386286..389216 2011-02-03 2011-02-03
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez)
Primary SGDIDS000000278
References cited on this page View Complete Literature Guide for PFF1
1) Hecht KA, et al.  (2013) Characterization of an M28 metalloprotease family member residing in the yeast vacuole. FEMS Yeast Res 13(5):471-84
2) Jonson L, et al.  (2004) Enhanced peptide secretion by gene disruption of CYM1, a novel protease in Saccharomyces cerevisiae. Eur J Biochem 271(23-24):4788-97