NTG1/YAL015C Summary Help

Standard Name NTG1 1
Systematic Name YAL015C
Alias FUN33 , SCR1 , ogg2 2 , 3
Feature Type ORF, Verified
Description DNA N-glycosylase and apurinic/apyrimidinic (AP) lyase; involved in base excision repair; acts in both nucleus and mitochondrion; creates a double-strand break at mtDNA origins that stimulates replication in response to oxidative stress; required for maintaining mitochondrial genome integrity; NTG1 has a paralog, NTG2, that arose from the whole genome duplication (4, 5, 6, 7, 8, 9)
Name Description eNdonuclease Three-like Glycosylase 1
Chromosomal Location
ChrI:128102 to 126903 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All NTG1 GO evidence and references
  View Computational GO annotations for NTG1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 3 genes
Resources
Classical genetics
null
Large-scale survey
null
Resources
65 total interaction(s) for 39 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 14
  • Affinity Capture-RNA: 3
  • Affinity Capture-Western: 1
  • Co-purification: 1

Genetic Interactions
  • Dosage Rescue: 1
  • Negative Genetic: 1
  • Phenotypic Enhancement: 14
  • Phenotypic Suppression: 8
  • Positive Genetic: 2
  • Synthetic Growth Defect: 12
  • Synthetic Lethality: 7
  • Synthetic Rescue: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 399
Molecular Weight (Da) 45,577
Isoelectric Point (pI) 8.06
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrI:128102 to 126903 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1200 128102..126903 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000013
References cited on this page View Complete Literature Guide for NTG1
1) Eide L, et al.  (1996) Base excision of oxidative purine and pyrimidine DNA damage in Saccharomyces cerevisiae by a DNA glycosylase with sequence similarity to endonuclease III from Escherichia coli. Proc Natl Acad Sci U S A 93(20):10735-40
2) Nash HM, et al.  (1996) Cloning of a yeast 8-oxoguanine DNA glycosylase reveals the existence of a base-excision DNA-repair protein superfamily. Curr Biol 6(8):968-80
3) Bruner SD, et al.  (1998) Repair of oxidatively damaged guanine in Saccharomyces cerevisiae by an alternative pathway. Curr Biol 8(7):393-403
4) Alseth I, et al.  (1999) The Saccharomyces cerevisiae homologues of endonuclease III from Escherichia coli, Ntg1 and Ntg2, are both required for efficient repair of spontaneous and induced oxidative DNA damage in yeast. Mol Cell Biol 19(5):3779-87
5) Meadows KL, et al.  (2003) Characterization of AP lyase activities of Saccharomyces cerevisiae Ntg1p and Ntg2p: implications for biological function. Nucleic Acids Res 31(19):5560-7
6) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
7) Phadnis N, et al.  (2006) Ntg1p, the base excision repair protein, generates mutagenic intermediates in yeast mitochondrial DNA. DNA Repair (Amst) 5(7):829-39
8) Griffiths LM, et al.  (2009) Dynamic compartmentalization of base excision repair proteins in response to nuclear and mitochondrial oxidative stress. Mol Cell Biol 29(3):794-807
9) Hori A, et al.  (2009) Reactive oxygen species regulate DNA copy number in isolated yeast mitochondria by triggering recombination-mediated replication. Nucleic Acids Res 37(3):749-61