SUP45/YBR143C Summary Help

SUP45 BASIC INFORMATION

Standard Name SUP45
Systematic Name YBR143C
Alias SAL4 , SUP1 , SUP47
Feature Type ORF, Verified
Description Polypeptide release factor (eRF1) in translation termination; mutant form acts as a recessive omnipotent suppressor; methylated by Mtq2p-Trm112p in ternary complex eRF1-eRF3-GTP; mutation of methylation site confers resistance to zymocin (1, 2, 3, 4, 5)
Name Description SUPpressor
Gene Product Alias eRF1
GO Annotations All SUP45 GO evidence and references
    View Computational GO annotations for SUP45
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Mutant Phenotype All SUP45 Phenotype details and references
Classical genetics
reduction of function
Large-scale survey
null
Interactions SUP45 All interactions details and references
77 total interaction(s) for 39 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 41
  • Affinity Capture-Western: 7
  • Biochemical Activity: 2
  • Reconstituted Complex: 6
  • Two-hybrid: 5
Genetic Interactions
  • Dosage Rescue: 9
  • Phenotypic Enhancement: 3
  • Synthetic Growth Defect: 2
  • Synthetic Rescue: 2
Sequence Information
ChrII:532176 to 530863 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Genetic position: 81 cM
Last Update Coordinates: 2004-07-16 | Sequence: 1997-01-28
Subfeature details
Relative
Coordinates		 Chromosomal
Coordinates		 Most Recent Updates
Coordinates Sequence
CDS 1..1314 532176..530863 2004-07-16 1997-01-28
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | UniProtKB
Primary SGDIDS000000347

SUP45 RESOURCES

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SGD ORF mapGBrowse
SGD ORF map
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  • Localization Resources
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  • Functional Analysis

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Expression Summary histogram

ADDITIONAL INFORMATION for SUP45

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for SUP45]

1) Stansfield I, et al.  (1992) Ribosomal association of the yeast SAL4 (SUP45) gene product: implications for its role in translation fidelity and termination. Mol Microbiol 6(23):3469-78
2) Himmelfarb HJ, et al.  (1985) Isolation of the SUP45 omnipotent suppressor gene of Saccharomyces cerevisiae and characterization of its gene product. Mol Cell Biol 5(4):816-22
3) Stansfield I, et al.  (1995) The products of the SUP45 (eRF1) and SUP35 genes interact to mediate translation termination in Saccharomyces cerevisiae. EMBO J 14(17):4365-73
4) Heurgue-Hamard V, et al.  (2006) The Zinc Finger Protein Ynr046w Is Plurifunctional and a Component of the eRF1 Methyltransferase in Yeast. J Biol Chem 281(47):36140-8
5) Studte P, et al.  (2008) tRNA and protein methylase complexes mediate zymocin toxicity in yeast. Mol Microbiol 69(5):1266-77