TRM10/YOL093W Summary Help

Standard Name TRM10 1
Systematic Name YOL093W
Feature Type ORF, Verified
Description tRNA methyltransferase; methylates the N-1 position of guanine at position 9 in tRNAs; protein abundance increases in response to DNA replication stress; member of the SPOUT (SpoU-TrmD) methyltransferase family; human ortholog TRMT10A plays a role in the pathogenesis of microcephaly and early onset diabetes; an 18-mer originates from the TRM10 locus; genetic analysis shows the 18-mer is the translation regulator (1, 2, 3, 4, 5)
Name Description Transfer RNA Methyltransferase 1
Chromosomal Location
ChrXV:142815 to 143696 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All TRM10 GO evidence and references
  View Computational GO annotations for TRM10
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
High-throughput
Regulators 3 genes
Resources
Classical genetics
null
unspecified
Large-scale survey
null
Resources
48 total interaction(s) for 42 unique genes/features.
Physical Interactions
  • Affinity Capture-RNA: 3
  • Biochemical Activity: 5

Genetic Interactions
  • Dosage Lethality: 2
  • Negative Genetic: 21
  • Phenotypic Enhancement: 1
  • Positive Genetic: 7
  • Synthetic Growth Defect: 7
  • Synthetic Lethality: 1
  • Synthetic Rescue: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 293
Molecular Weight (Da) 34,520
Isoelectric Point (pI) 10.03
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXV:142815 to 143696 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2006-01-05 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..882 142815..143696 2006-01-05 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005453
References cited on this page View Complete Literature Guide for TRM10
1) Jackman JE, et al.  (2003) Identification of the yeast gene encoding the tRNA m1G methyltransferase responsible for modification at position 9. RNA 9(5):574-85
2) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
3) Shao Z, et al.  (2014) Crystal structure of tRNA m1G9 methyltransferase Trm10: insight into the catalytic mechanism and recognition of tRNA substrate. Nucleic Acids Res 42(1):509-25
4) Igoillo-Esteve M, et al.  (2013) tRNA Methyltransferase Homolog Gene TRMT10A Mutation in Young Onset Diabetes and Primary Microcephaly in Humans. PLoS Genet 9(10):e1003888
5) Pircher A, et al.  (2014) An mRNA-Derived Noncoding RNA Targets and Regulates the Ribosome. Mol Cell 54(1):147-55