STE6/YKL209C Summary Help

Standard Name STE6
Systematic Name YKL209C
Feature Type ORF, Verified
Description Plasma membrane ATP-binding cassette (ABC) transporter; required for the export of a-factor, catalyzes ATP hydrolysis coupled to a-factor transport; contains 12 transmembrane domains and two ATP binding domains; expressed only in MATa cells (1, 2, 3, 4, 5 and see Summary Paragraph)
Name Description STErile
Chromosomal Location
ChrXI:46295 to 42423 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Genetic position: -152.3 cM
Gene Ontology Annotations All STE6 GO evidence and references
  View Computational GO annotations for STE6
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 11 genes
Resources
Classical genetics
null
Large-scale survey
null
overexpression
Resources
33 total interaction(s) for 24 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 7
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 12
  • PCA: 3
  • Reconstituted Complex: 1
  • Two-hybrid: 2

Genetic Interactions
  • Dosage Rescue: 1
  • Synthetic Growth Defect: 2
  • Synthetic Lethality: 3
  • Synthetic Rescue: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 1,290
Molecular Weight (Da) 144,764
Isoelectric Point (pI) 7.91
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXI:46295 to 42423 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Genetic position: -152.3 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..3873 46295..42423 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | TCDB | UniProtKB
Primary SGDIDS000001692
SUMMARY PARAGRAPH for STE6

STE6 was first identified as a gene necessary for mating in MAT a cells (6, 7). The Ste6 protein is a membrane transporter of the ABC (ATP-binding cassette) family. While alpha factor is exported in MATalpha cells via the classical secretion pathway, a-factor is pumped out of the cell by the MATa cell-specific protein Ste6p (3, 1). In MATalpha cells, STE6 expression is repressed by the Alpha2 gene product (7, 8). Ste6p is related to human MDR1, a P-glycoprotein/multiple drug resistance protein (1, 3); several MDR proteins, including mouse Mdr3 and cystic fibrosis transmembrane conductance regulator (CFTR)/Ste6 chimeras, can complement ste6 null mutations in vivo in yeast (9, 10). In fact, a model has been established by these cross-species complementation tests to study the function of higher eukaryotic MDR proteins (11).

Last updated: 1999-03-31 Contact SGD

References cited on this page View Complete Literature Guide for STE6
1) McGrath JP and Varshavsky A  (1989) The yeast STE6 gene encodes a homologue of the mammalian multidrug resistance P-glycoprotein. Nature 340(6232):400-4
2) Michaelis S  (1993) STE6, the yeast a-factor transporter. Semin Cell Biol 4(1):17-27
3) Kuchler K, et al.  (1989) Saccharomyces cerevisiae STE6 gene product: a novel pathway for protein export in eukaryotic cells. EMBO J 8(13):3973-84
4) Loayza D and Michaelis S  (1998) Role for the ubiquitin-proteasome system in the vacuolar degradation of Ste6p, the a-factor transporter in Saccharomyces cerevisiae. Mol Cell Biol 18(2):779-89
5) Ketchum CJ, et al.  (2001) The yeast a-factor transporter Ste6p, a member of the ABC superfamily, couples ATP hydrolysis to pheromone export. J Biol Chem 276(31):29007-11
6) Chan RK, et al.  (1983) Extracellular suppression allows mating by pheromone-deficient sterile mutants of Saccharomyces cerevisiae. J Bacteriol 155(2):903-6
7) Wilson KL and Herskowitz I  (1984) Negative regulation of STE6 gene expression by the alpha 2 product of Saccharomyces cerevisiae. Mol Cell Biol 4(11):2420-7
8) Wilson KL and Herskowitz I  (1986) Sequences upstream of the STE6 gene required for its expression and regulation by the mating type locus in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A 83(8):2536-40
9) Raymond M, et al.  (1992) Functional complementation of yeast ste6 by a mammalian multidrug resistance mdr gene. Science 256(5054):232-4
10) Teem JL, et al.  (1993) Identification of revertants for the cystic fibrosis delta F508 mutation using STE6-CFTR chimeras in yeast. Cell 73(2):335-46
11) Hemenway CS and Heitman J  (1996) Immunosuppressant target protein FKBP12 is required for P-glycoprotein function in yeast. J Biol Chem 271(31):18527-34