SGF73/YGL066W Summary Help

Standard Name SGF73 1
Systematic Name YGL066W
Alias SCA7 2
Feature Type ORF, Verified
Description SAGA complex subunit; has a role in anchoring the deubiquitination module into SAGA and SLIK complexes; involved in preinitiation complex assembly at promoters; relocalizes to the cytosol in response to hypoxia; human ortholog ataxin-7 is associated with spinocerebellar ataxia diseases; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay (1, 2, 3, 4, 5, 6, 7, 8)
Name Description SaGa associated Factor, 73 kDa 1
Chromosomal Location
ChrVII:377609 to 379582 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All SGF73 GO evidence and references
  View Computational GO annotations for SGF73
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 2 genes
Resources
Classical genetics
null
overexpression
Large-scale survey
null
overexpression
Resources
625 total interaction(s) for 355 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 208
  • Affinity Capture-RNA: 4
  • Affinity Capture-Western: 21
  • Biochemical Activity: 6
  • Reconstituted Complex: 3
  • Two-hybrid: 5

Genetic Interactions
  • Dosage Rescue: 1
  • Negative Genetic: 274
  • Phenotypic Suppression: 1
  • Positive Genetic: 43
  • Synthetic Growth Defect: 51
  • Synthetic Lethality: 7
  • Synthetic Rescue: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 657
Molecular Weight (Da) 72,878
Isoelectric Point (pI) 9.57
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrVII:377609 to 379582 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1974 377609..379582 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003034
References cited on this page View Complete Literature Guide for SGF73
1) Sanders SL, et al.  (2002) Proteomics of the eukaryotic transcription machinery: identification of proteins associated with components of yeast TFIID by multidimensional mass spectrometry. Mol Cell Biol 22(13):4723-38
2) McMahon SJ, et al.  (2005) Polyglutamine-expanded spinocerebellar ataxia-7 protein disrupts normal SAGA and SLIK histone acetyltransferase activity. Proc Natl Acad Sci U S A 102(24):8478-82
3) Shukla A, et al.  (2006) SAGA-associated Sgf73p facilitates formation of the preinitiation complex assembly at the promoters either in a HAT-dependent or independent manner in vivo. Nucleic Acids Res 34(21):6225-32
4) Helmlinger D, et al.  (2004) Ataxin-7 is a subunit of GCN5 histone acetyltransferase-containing complexes. Hum Mol Genet 13(12):1257-65
5) Jordan PW, et al.  (2007) Novel roles for selected genes in meiotic DNA processing. PLoS Genet 3(12):e222
6) Lee KK, et al.  (2009) Yeast Sgf73/Ataxin-7 serves to anchor the deubiquitination module into both SAGA and Slik(SALSA) HAT complexes. Epigenetics Chromatin 2(1):2
7) Tous C, et al.  (2011) A novel assay identifies transcript elongation roles for the Nup84 complex and RNA processing factors. EMBO J 30(10):1953-64
8) Ghosh Dastidar R, et al.  (2012) The nuclear localization of SWI/SNF proteins is subjected to oxygen regulation. Cell Biosci 2(1):30