RPT1/YKL145W Summary Help

Standard Name RPT1 1
Systematic Name YKL145W
Alias CIM5 2 , YTA3 3
Feature Type ORF, Verified
Description ATPase of the 19S regulatory particle of the 26S proteasome; one of six ATPases of the regulatory particle; involved in the degradation of ubiquitinated substrates; required for optimal CDC20 transcription; interacts with Rpn12p and Ubr1p; mutant has aneuploidy tolerance (4, 5, 6, 7, 8)
Name Description Regulatory Particle Triple-A protein, or Regulatory Particle Triphosphatase 1
Chromosomal Location
ChrXI:174213 to 175616 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All RPT1 GO evidence and references
  View Computational GO annotations for RPT1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 5 genes
Resources
Classical genetics
conditional
null
reduction of function
Large-scale survey
null
reduction of function
Resources
383 total interaction(s) for 120 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 160
  • Affinity Capture-RNA: 3
  • Affinity Capture-Western: 110
  • Co-crystal Structure: 2
  • Co-fractionation: 3
  • Co-localization: 1
  • Co-purification: 22
  • PCA: 7
  • Reconstituted Complex: 14
  • Two-hybrid: 4

Genetic Interactions
  • Dosage Rescue: 2
  • Negative Genetic: 14
  • Phenotypic Enhancement: 2
  • Positive Genetic: 32
  • Synthetic Growth Defect: 4
  • Synthetic Lethality: 2
  • Synthetic Rescue: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 467
Molecular Weight (Da) 51,982
Isoelectric Point (pI) 5.17
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXI:174213 to 175616 | ORF Map | GBrowse
This feature contains embedded feature(s): YKL145W-A
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1404 174213..175616 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001628
References cited on this page View Complete Literature Guide for RPT1
1) Finley D, et al.  (1998) Unified nomenclature for subunits of the Saccharomyces cerevisiae proteasome regulatory particle. Trends Biochem Sci 23(7):244-5
2) Ghislain M, et al.  (1993) S. cerevisiae 26S protease mutants arrest cell division in G2/metaphase. Nature 366(6453):358-62
3) Schnall R, et al.  (1994) Identification of a set of yeast genes coding for a novel family of putative ATPases with high similarity to constituents of the 26S protease complex. Yeast 10(9):1141-55
4) Takeuchi J and Toh-e A  (1999) Genetic evidence for interaction between components of the yeast 26S proteasome: combination of a mutation in RPN12 (a lid component gene) with mutations in RPT1 (an ATPase gene) causes synthetic lethality. Mol Gen Genet 262(1):145-53
5) Glickman MH, et al.  (1998) The regulatory particle of the Saccharomyces cerevisiae proteasome. Mol Cell Biol 18(6):3149-62
6) Rubin DM, et al.  (1998) Active site mutants in the six regulatory particle ATPases reveal multiple roles for ATP in the proteasome. EMBO J 17(17):4909-19
7) Morris MC, et al.  (2003) Cks1-dependent proteasome recruitment and activation of CDC20 transcription in budding yeast. Nature 423(6943):1009-13
8) Torres EM, et al.  (2010) Identification of aneuploidy-tolerating mutations. Cell 143(1):71-83