RNT1/YMR239C Summary Help

Standard Name RNT1 1
Systematic Name YMR239C
Feature Type ORF, Verified
Description Nuclear dsRNA-specific ribonuclease (RNase III); involved in rDNA transcription, rRNA processing and U2 snRNA 3' end formation by cleavage of a stem-loop structure at the 3' end of U2 snRNA; involved in polyadenylation-independent transcription termination; involved in the cell wall stress response, regulating the degradation of cell wall integrity and morphogenesis checkpoint genes (1, 2, 3, 4, 5)
Name Description RNase Three 1
Chromosomal Location
ChrXIII:749677 to 748262 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gene Ontology Annotations All RNT1 GO evidence and references
  View Computational GO annotations for RNT1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 2 genes
Classical genetics
Large-scale survey
reduction of function
62 total interaction(s) for 48 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 12
  • Affinity Capture-RNA: 3
  • Affinity Capture-Western: 3
  • Biochemical Activity: 9
  • PCA: 1
  • Protein-RNA: 2
  • Reconstituted Complex: 3
  • Two-hybrid: 8

Genetic Interactions
  • Dosage Growth Defect: 2
  • Dosage Lethality: 2
  • Dosage Rescue: 1
  • Negative Genetic: 2
  • Phenotypic Enhancement: 1
  • Phenotypic Suppression: 2
  • Synthetic Growth Defect: 4
  • Synthetic Lethality: 7

Expression Summary
Length (a.a.) 471
Molecular Weight (Da) 54,070
Isoelectric Point (pI) 9.32
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrXIII:749677 to 748262 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..1416 749677..748262 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004852
References cited on this page View Complete Literature Guide for RNT1
1) Elela SA, et al.  (1996) RNase III cleaves eukaryotic preribosomal RNA at a U3 snoRNP-dependent site. Cell 85(1):115-24
2) Abou Elela S and Ares M Jr  (1998) Depletion of yeast RNase III blocks correct U2 3' end formation and results in polyadenylated but functional U2 snRNA. EMBO J 17(13):3738-46
3) Catala M, et al.  (2008) Deletion of Rnt1p alters the proportion of open versus closed rRNA gene repeats in yeast. Mol Cell Biol 28(2):619-29
4) Ghazal G, et al.  (2009) Yeast RNase III triggers polyadenylation-independent transcription termination. Mol Cell 36(1):99-109
5) Catala M, et al.  (2012) RNA-dependent regulation of the cell wall stress response. Nucleic Acids Res 40(15):7507-17