PAB1/YER165W Summary Help

Standard Name PAB1 1
Systematic Name YER165W
Feature Type ORF, Verified
Description Poly(A) binding protein; part of the 3'-end RNA-processing complex, mediates interactions between the 5' cap structure and the 3' mRNA poly(A) tail, involved in control of poly(A) tail length, interacts with translation factor eIF-4G (2, 3, 4)
Name Description Poly(A) Binding protein 5
Chromosomal Location
ChrV:510373 to 512106 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All PAB1 GO evidence and references
  View Computational GO annotations for PAB1
Molecular Function
Manually curated
High-throughput
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 8 genes
Resources
Classical genetics
conditional
null
reduction of function
repressible
Large-scale survey
null
overexpression
repressible
Resources
368 total interaction(s) for 231 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 199
  • Affinity Capture-RNA: 4
  • Affinity Capture-Western: 48
  • Biochemical Activity: 4
  • Co-fractionation: 9
  • Co-localization: 1
  • Co-purification: 1
  • PCA: 2
  • Reconstituted Complex: 21
  • Two-hybrid: 11

Genetic Interactions
  • Dosage Growth Defect: 1
  • Dosage Lethality: 1
  • Dosage Rescue: 12
  • Negative Genetic: 20
  • Positive Genetic: 9
  • Synthetic Growth Defect: 1
  • Synthetic Lethality: 7
  • Synthetic Rescue: 17

Resources
Expression Summary
histogram
Resources
Length (a.a.) 577
Molecular Weight (Da) 64,344
Isoelectric Point (pI) 5.83
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrV:510373 to 512106 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1734 510373..512106 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000967
References cited on this page View Complete Literature Guide for PAB1
1) Proweller A and Butler S  (1994) Efficient translation of poly(A)-deficient mRNAs in Saccharomyces cerevisiae. Genes Dev 8(21):2629-40
2) Kessler SH and Sachs AB  (1998) RNA recognition motif 2 of yeast Pab1p is required for its functional interaction with eukaryotic translation initiation factor 4G. Mol Cell Biol 18(1):51-7
3) Wyers F, et al.  (2000) Deletion of the PAT1 gene affects translation initiation and suppresses a PAB1 gene deletion in yeast. Mol Cell Biol 20(10):3538-49
4) Amrani N, et al.  (1997) Yeast Pab1 interacts with Rna15 and participates in the control of the poly(A) tail length in vitro. Mol Cell Biol 17(7):3694-701
5) Sachs AB, et al.  (1987) A single domain of yeast poly(A)-binding protein is necessary and sufficient for RNA binding and cell viability. Mol Cell Biol 7(9):3268-76