NUP42/YDR192C Summary Help

Standard Name NUP42 1
Systematic Name YDR192C
Alias RIP1 1 , UIP1 2
Feature Type ORF, Verified
Description FG-nucleoporin component of central core of the nuclear pore complex; also part of the nuclear pore complex (NPC) cytoplasmic filaments; contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier and is involved in gene tethering at the nuclear periphery; interacts with Gle1p (3, 4, 5, 6, 7, 8 and see Summary Paragraph)
Name Description NUclear Pore 9
Chromosomal Location
ChrIV:844861 to 843569 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All NUP42 GO evidence and references
  View Computational GO annotations for NUP42
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 2 genes
Resources
Classical genetics
null
Large-scale survey
null
overexpression
Resources
160 total interaction(s) for 84 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 50
  • Affinity Capture-RNA: 3
  • Co-purification: 1
  • Reconstituted Complex: 9
  • Two-hybrid: 22

Genetic Interactions
  • Dosage Rescue: 4
  • Negative Genetic: 37
  • Phenotypic Enhancement: 2
  • Phenotypic Suppression: 2
  • Positive Genetic: 2
  • Synthetic Growth Defect: 13
  • Synthetic Lethality: 11
  • Synthetic Rescue: 4

Resources
Expression Summary
histogram
Resources
Length (a.a.) 430
Molecular Weight (Da) 42,778
Isoelectric Point (pI) 10.73
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrIV:844861 to 843569 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1293 844861..843569 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002600
SUMMARY PARAGRAPH for NUP42

NUP42 encodes a nuclear pore protein first identified as interacting with the effector domain of HIV-1 Rev expressed in yeast (10, 11). Transport of macromolecules between the nucleus and the cytoplasm of eukaryotic cells occurs through the nuclear pore complex (NPC), a large macromolecular complex that spans the nuclear envelope (reviewed in 11, 12, 13, 14). The structure of the vertebrate NPC has been studied extensively; recent reviews include 15, 16, 17, and 18. The yeast NPC shares several features with the vertebrate NPC, despite being smaller and less elaborate (19, 20). Many yeast nuclear pore proteins, or nucleoporins, have been identified by a variety of genetic approaches (reviewed in 11, 12, 21, 22, 23). Nup42p interacts with the RNA export factor Gle1p (24) and with the nuclear pore proteins Nup100p and Gle2p (11). Nup42p is not essential for viability, but is required for the export of heat shock mRNA following stress (25, 11). Nup42p contains FG repeats (11), but deleting the repeats has no effect on mRNA export (26).

Last updated: 1999-07-27 Contact SGD

References cited on this page View Complete Literature Guide for NUP42
1) SGD  (2009) Gene name standardized from a GenBank/EMBL/DDBJ record
2) Takahashi Y, et al.  (2000) Yeast Ulp1, an Smt3-specific protease, associates with nucleoporins. J Biochem 128(5):723-5
3) Strahm Y, et al.  (1999) The RNA export factor Gle1p is located on the cytoplasmic fibrils of the NPC and physically interacts with the FG-nucleoporin Rip1p, the DEAD-box protein Rat8p/Dbp5p and a new protein Ymr 255p. EMBO J 18(20):5761-77
4) Rout MP, et al.  (2000) The yeast nuclear pore complex: composition, architecture, and transport mechanism. J Cell Biol 148(4):635-51
5) Allen NP, et al.  (2001) Proteomic analysis of nucleoporin interacting proteins. J Biol Chem 276(31):29268-74
6) Patel SS, et al.  (2007) Natively unfolded nucleoporins gate protein diffusion across the nuclear pore complex. Cell 129(1):83-96
7) Light WH, et al.  (2010) Interaction of a DNA Zip code with the nuclear pore complex promotes H2A.Z incorporation and INO1 transcriptional memory. Mol Cell 40(1):112-25
8) Bradatsch B, et al.  (2007) Arx1 functions as an unorthodox nuclear export receptor for the 60S preribosomal subunit. Mol Cell 27(5):767-79
9) Davis LI and Fink GR  (1990) The NUP1 gene encodes an essential component of the yeast nuclear pore complex. Cell 61(6):965-78
10) Stutz F, et al.  (1995) Identification of a novel nuclear pore-associated protein as a functional target of the HIV-1 Rev protein in yeast. Cell 82(3):495-506
11) Fabre E and Hurt E  (1997) Yeast genetics to dissect the nuclear pore complex and nucleocytoplasmic trafficking. Annu Rev Genet 31:277-313
12) Wente SR, et al.  (1997) "The nucleus and nucleocytoplasmic transport in Saccharomyces cerevisiae." Pp. 471-546 in The Molecular and Cellular Biology of the Yeast Saccharomyces: Cell Cycle and Cell Biology, edited by Pringle JR, Broach JR and Jones EW. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press
13) Pemberton LF, et al.  (1998) Transport routes through the nuclear pore complex. Curr Opin Cell Biol 10(3):392-9
14) Izaurralde E and Adam S  (1998) Transport of macromolecules between the nucleus and the cytoplasm. RNA 4(4):351-64
15) Hinshaw JE  (1994) Architecture of the nuclear pore complex and its involvement in nucleocytoplasmic transport. Biochem Pharmacol 47(1):15-20
16) Pante N and Aebi U  (1996) Molecular dissection of the nuclear pore complex. Crit Rev Biochem Mol Biol 31(2):153-99
17) Davis LI  (1995) The nuclear pore complex. Annu Rev Biochem 64:865-96
18) Pante N and Aebi U  (1994) Toward the molecular details of the nuclear pore complex. J Struct Biol 113(3):179-89
19) Rout MP and Blobel G  (1993) Isolation of the yeast nuclear pore complex. J Cell Biol 123(4):771-83
20) Yang Q, et al.  (1998) Three-dimensional architecture of the isolated yeast nuclear pore complex: functional and evolutionary implications. Mol Cell 1(2):223-34
21) Doye V and Hurt E  (1997) From nucleoporins to nuclear pore complexes. Curr Opin Cell Biol 9(3):401-11
22) Doye V and Hurt EC  (1995) Genetic approaches to nuclear pore structure and function. Trends Genet 11(6):235-41
23) Newmeyer DD  (1993) The nuclear pore complex and nucleocytoplasmic transport. Curr Opin Cell Biol 5(3):395-407
24) Murphy R and Wente SR  (1996) An RNA-export mediator with an essential nuclear export signal. Nature 383(6598):357-60
25) Saavedra CA, et al.  (1997) Yeast heat shock mRNAs are exported through a distinct pathway defined by Rip1p. Genes Dev 11(21):2845-56
26) Stutz F, et al.  (1997) The yeast nucleoporin rip1p contributes to multiple export pathways with no essential role for its FG-repeat region. Genes Dev 11(21):2857-68