NUC1/YJL208C Summary Help

Standard Name NUC1 1
Systematic Name YJL208C
Feature Type ORF, Verified
Description Major mitochondrial nuclease; has RNAse and DNA endo- and exonucleolytic activities; roles in mitochondrial recombination, apoptosis and maintenance of polyploidy; involved in fragmentation of genomic DNA during PND (programmed nuclear destruction); encodes ortholog of mammalian endoG (1, 2, 3, 4, 5, 6)
Name Description NUClease 1
Chromosomal Location
ChrX:41183 to 40194 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All NUC1 GO evidence and references
  View Computational GO annotations for NUC1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 148 genes
Resources
Classical genetics
null
overexpression
Large-scale survey
null
overexpression
Resources
58 total interaction(s) for 45 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 22
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 2

Genetic Interactions
  • Negative Genetic: 23
  • Phenotypic Enhancement: 1
  • Phenotypic Suppression: 1
  • Positive Genetic: 1
  • Synthetic Growth Defect: 3
  • Synthetic Lethality: 3
  • Synthetic Rescue: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 329
Molecular Weight (Da) 37,209
Isoelectric Point (pI) 9.2
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrX:41183 to 40194 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 1996-07-31 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..990 41183..40194 1996-07-31 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003744
References cited on this page View Complete Literature Guide for NUC1
1) Vincent RD, et al.  (1988) Sequence and expression of NUC1, the gene encoding the mitochondrial nuclease in Saccharomyces cerevisiae. Nucleic Acids Res 16(8):3297-312
2) Zassenhaus HP and Denniger G  (1994) Analysis of the role of the NUC1 endo/exonuclease in yeast mitochondrial DNA recombination. Curr Genet 25(2):142-9
3) Dake E, et al.  (1988) Purification and properties of the major nuclease from mitochondria of Saccharomyces cerevisiae. J Biol Chem 263(16):7691-702
4) Buttner S, et al.  (2007) Endonuclease g regulates budding yeast life and death. Mol Cell 25(2):233-46
5) Buttner S, et al.  (2007) Depletion of endonuclease G selectively kills polyploid cells. Cell Cycle 6(9):1072-6
6) Eastwood MD, et al.  (2012) Developmentally programmed nuclear destruction during yeast gametogenesis. Dev Cell 23(1):35-44