LYS2/YBR115C Summary Help

LYS2 BASIC INFORMATION

Standard Name LYS2 1
Systematic Name YBR115C
Feature Type ORF, Verified
Description Alpha aminoadipate reductase, catalyzes the reduction of alpha-aminoadipate to alpha-aminoadipate 6-semialdehyde, which is the fifth step in biosynthesis of lysine; activation requires posttranslational phosphopantetheinylation by Lys5p (2 and see Summary Paragraph)
Name Description LYSine requiring
GO Annotations All LYS2 GO evidence and references
    View Computational GO annotations for LYS2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Pathways
Mutant Phenotype All LYS2 Phenotype details and references
Classical genetics
reduction of function
Large-scale survey
null
Interactions LYS2 All interactions details and references
15 total interaction(s) for 13 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 9
Genetic Interactions
  • Synthetic Rescue: 6
Sequence Information
ChrII:473920 to 469742 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Genetic position: 59 cM
Last Update Coordinates: 2004-07-16 | Sequence: 1997-01-28
Subfeature details
Relative
Coordinates		 Chromosomal
Coordinates		 Most Recent Updates
Coordinates Sequence
CDS 1..4179 473920..469742 2004-07-16 1997-01-28
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | UniProtKB
Primary SGDIDS000000319

LYS2 RESOURCES

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SGD ORF map
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  • Functional Analysis

Click on histogram for expression summary
Expression Summary histogram

ADDITIONAL INFORMATION for LYS2

SUMMARY PARAGRAPH for LYS2

About lysine biosynthesis

S. cerevisiae synthesizes the essential amino acid L-lysine via the L-alpha-aminoadipic acid pathway instead of the diaminopmelate pathway (3). Originally proposed to be characteristic of fungi, recent studies suggest prokaryotes also synthesize lysine via the alpha-aminoadipic acid pathway (4). Intermediates in this pathway are often incorporated into secondary metabolites. For example, it has been well- studied that alpha-aminoadipate is required for penicillin production (3). Regulation of the lysine biosynthetic pathway in S. cerevisiae is an interaction between general amino acid control (via Gcn4p) (5), feedback inhibition of homocitrate synthase activity by lysine (6), and induction of Lys14p by alpha-aminoadipate semialdehyde (7).

Last updated: 2007-10-04

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for LYS2]

1) Plotkin, D.J.  (1978) Commitment to meiotic recombination: a temporal analysis. Ph.D Thesis
2) Sinha AK and Bhattacharjee JK  (1971) Lysine biosynthesis in Saccharomyces. Conversion of -aminoadipate into -aminoadipic -semialdehyde. Biochem J 125(3):743-9
3) Zabriskie TM and Jackson MD  (2000) Lysine biosynthesis and metabolism in fungi. Nat Prod Rep 17(1):85-97
4) Nishida H and Nishiyama M  (2000) What is characteristic of fungal lysine synthesis through the alpha-aminoadipate pathway? J Mol Evol 51(3):299-302
5) Hinnebusch A  (1992) "General and Pathway-specific Regulatory Mechanisms Controlling the Synthesis of Amino Acid Biosynthetic Enzymes in Saccharomyces cerevisiae". Pp. 319-414 in The Molecular and Cellular Biology of the Yeast Saccharomyces: Gene Expression, edited by Jones EW, Pringle JR and Broach JR. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press
6) Feller A, et al.  (1999) In Saccharomyces cerevisae, feedback inhibition of homocitrate synthase isoenzymes by lysine modulates the activation of LYS gene expression by Lys14p. Eur J Biochem 261(1):163-70
7) El Alami M, et al.  (2000) Characterisation of a tripartite nuclear localisation sequence in the regulatory protein Lys14 of Saccharomyces cerevisiae. Curr Genet 38(2):78-86