HIS3/YOR202W Summary 
HIS3 BASIC INFORMATION
| Standard Name | HIS3 1 |
|---|---|
| Systematic Name | YOR202W |
| Alias | HIS10 2 , HIS8 2 |
| Feature Type | ORF, Verified |
| Description | Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p (1, 2, 3, 4, 5 and see Summary Paragraph) 
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| Name Description | HIStidine 1 |
| GO Annotations | All HIS3 GO evidence and references |
|---|---|
| View Computational GO annotations for HIS3 | |
| Molecular Function | |
| Manually curated | |
| Biological Process | |
| Manually curated | |
| Cellular Component | |
| Manually curated |
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| Pathways |
|---|
| Mutant Phenotype | All HIS3 Phenotype details and references |
|---|---|
| Classical genetics | |
| null | |
| unspecified | |
| Large-scale survey | |
| null |
| Interactions | HIS3 All interactions details and references |
|---|---|
| 2 total interaction(s) for 2 unique genes/features. | |
| Physical Interactions |
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| External Links | All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | UniProtKB |
|---|
| Primary SGDID | S000005728 |
|---|
HIS3 RESOURCES
| SGD ORF map | GBrowse |
|---|---|
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Click on histogram for expression summary
Expression Summary
ADDITIONAL INFORMATION for HIS3
SUMMARY PARAGRAPH for HIS3
HIS3 encodes imidazoleglycerol-phosphate dehydratase, which catalyzes the sixth step in histidine biosynthesis (5). The biosynthesis of histidine has been most extensively studied in Salmonella typhimurium and E. coli. The reactions and enzymes involved in histidine biosynthesis have been identified in many organisms, and are thoroughly reviewed in Alifano et al. (5). Mutations in HIS3, as well as in genes encoding other histidine biosynthetic enzymes, cause histidine auxotrophy and sensitivity to copper, cobalt, and nickel salts (4). Transcription of HIS3 is regulated by general amino acid control, in which the transcription factor Gcn4p plays a key role (reviewed in 6). HIS3 has been widely used in studies of transcriptional regulation (6) and as a selectable marker for plasmid constructs (for example, see 7 and 8).
Last updated: 1999-11-10
REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for HIS3]
| 1) | Struhl K and Davis RW (1977) Production of a functional eukaryotic enzyme in Escherichia coli: cloning and expression of the yeast structural gene for imidazole-glycerolphosphate dehydratase (his3). Proc Natl Acad Sci U S A 74(12):5255-9 |
| 2) | FINK GR (1964) GENE-ENZYME RELATIONS IN HISTIDINE BIOSYNTHESIS IN YEAST. Science 146:525-7 |
| 3) | Hope IA and Struhl K (1985) GCN4 protein, synthesized in vitro, binds HIS3 regulatory sequences: implications for general control of amino acid biosynthetic genes in yeast. Cell 43(1):177-88 |
| 4) | Pearce DA and Sherman F (1999) Toxicity of copper, cobalt, and nickel salts is dependent on histidine metabolism in the yeast Saccharomyces cerevisiae. J Bacteriol 181(16):4774-9 |
| 5) | Alifano P, et al. (1996) Histidine biosynthetic pathway and genes: structure, regulation, and evolution. Microbiol Rev 60(1):44-69 |
| 6) | Hinnebusch A (1992) "General and Pathway-specific Regulatory Mechanisms Controlling the Synthesis of Amino Acid Biosynthetic Enzymes in Saccharomyces cerevisiae". Pp. 319-414 in The Molecular and Cellular Biology of the Yeast Saccharomyces: Gene Expression, edited by Jones EW, Pringle JR and Broach JR. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press |
| 7) | Baganz F, et al. (1997) Suitability of replacement markers for functional analysis studies in Saccharomyces cerevisiae. Yeast 13(16):1563-73 |
| 8) | Sikorski RS and Hieter P (1989) A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics 122(1):19-27 |
