HBS1/YKR084C Summary Help

Standard Name HBS1 1
Systematic Name YKR084C
Feature Type ORF, Verified
Description GTPase with similarity to translation release factors; together with binding partner Dom34p, facilitates ribosomal subunit dissociation and peptidyl-tRNA release when translation is stalled, particularly in 3' UTRs; genetically implicated in mRNA no-go decay; HBS1 has a paralog, SKI7, that arose from the whole genome duplication (1, 2, 3, 4, 5, 6, 7, 8)
Name Description Hsp70 subfamily B Suppressor 1
Chromosomal Location
ChrXI:598890 to 597055 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gene Ontology Annotations All HBS1 GO evidence and references
  View Computational GO annotations for HBS1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Regulators 2 genes
Classical genetics
Large-scale survey
47 total interaction(s) for 34 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 2
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 1
  • Biochemical Activity: 3
  • Co-crystal Structure: 5
  • Co-fractionation: 1
  • Co-purification: 1
  • Reconstituted Complex: 1
  • Two-hybrid: 3

Genetic Interactions
  • Negative Genetic: 18
  • Phenotypic Enhancement: 1
  • Positive Genetic: 2
  • Synthetic Growth Defect: 6
  • Synthetic Rescue: 1

Expression Summary
Length (a.a.) 611
Molecular Weight (Da) 68,729
Isoelectric Point (pI) 5.09
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrXI:598890 to 597055 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..1836 598890..597055 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001792
References cited on this page View Complete Literature Guide for HBS1
1) Nelson RJ, et al.  (1992) The translation machinery and 70 kd heat shock protein cooperate in protein synthesis. Cell 71(1):97-105
2) Carr-Schmid A, et al.  (2002) Novel G-protein complex whose requirement is linked to the translational status of the cell. Mol Cell Biol 22(8):2564-74
3) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
4) Doma MK and Parker R  (2006) Endonucleolytic cleavage of eukaryotic mRNAs with stalls in translation elongation. Nature 440(7083):561-4
5) Passos DO, et al.  (2009) Analysis of Dom34 and its function in no-go decay. Mol Biol Cell 20(13):3025-32
6) Shoemaker CJ, et al.  (2010) Dom34:Hbs1 promotes subunit dissociation and peptidyl-tRNA drop-off to initiate no-go decay. Science 330(6002):369-72
7) van den Elzen AM, et al.  (2014) Dom34-Hbs1 mediated dissociation of inactive 80S ribosomes promotes restart of translation after stress. EMBO J 33(3):265-76
8) Guydosh NR and Green R  (2014) Dom34 rescues ribosomes in 3' untranslated regions. Cell 156(5):950-62