GEF1/YJR040W Summary

GEF1 BASIC INFORMATION

Standard Name	GEF1
Systematic Name	YJR040W
Alias	CLC
Feature Type	ORF, Verified
Description	Voltage-gated chloride channel localized to the golgi, the endosomal system, and plasma membrane, and involved in cation homeostasis; highly homologous to vertebrate voltage-gated chloride channels (1, 2, 3)
Name Description	Glycerol Ethanol, Ferric requiring 4

GO Annotations	All GEF1 GO evidence and references
	View Computational GO annotations for GEF1
Molecular Function
Manually curated	voltage-gated chloride channel activity (IDA)
Biological Process
Manually curated	cellular copper ion homeostasis (IMP) cellular iron ion homeostasis (IMP)
Cellular Component
Manually curated	endoplasmic reticulum (IDA) endosome (IDA) fungal-type vacuole (IDA) Golgi apparatus (IDA) Golgi medial cisterna (IDA) plasma membrane (IDA)

Mutant Phenotype	All GEF1 Phenotype details and references
Classical genetics
null	chloride accumulation: decreased mitochondrial morphology: abnormal petite respiratory growth: absent vegetative growth: absent vegetative growth: decreased viable
unspecified	respiratory growth: normal rate respiratory growth: absent small molecule transport: increased
Large-scale survey
null	competitive fitness: decreased resistance to rapamycin: decreased resistance to wortmannin: normal resistance to doxorubicin: decreased respiratory growth: decreased rate respiratory growth: absent toxin resistance: decreased vegetative growth: decreased viable

Interactions	GEF1 All interactions details and references
	43 total interaction(s) for 27 unique genes/features.
Physical Interactions	Affinity Capture-MS: 2 Affinity Capture-Western: 2 PCA: 2 Two-hybrid: 5
Genetic Interactions	Phenotypic Enhancement: 20 Phenotypic Suppression: 5 Synthetic Growth Defect: 2 Synthetic Lethality: 4 Synthetic Rescue: 1

Sequence Information

ChrX:507738 to 510077 | |

Last Update

Coordinates: 2009-02-18 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..2340	507738..510077	2009-02-18	1996-07-31

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| TCDB \| UniProtKB

Primary SGDID	S000003801

GEF1 RESOURCES

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SGD ORF map	GBrowse

Click on histogram for expression summary
Expression Summary

ADDITIONAL INFORMATION for GEF1

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for GEF1]

1)	Gaxiola RA, et al. (1998) The yeast CLC chloride channel functions in cation homeostasis. Proc Natl Acad Sci U S A 95(7):4046-50
2)	Flis K, et al. (2002) The Gef1 protein of Saccharomyces cerevisiae is associated with chloride channel activity. Biochem Biophys Res Commun 294(5):1144-50
3)	Lopez-Rodriguez A, et al. (2007) The product of the gene GEF1 of Saccharomyces cerevisiae transports Cl across the plasma membrane. FEMS Yeast Res 7(8):1218-29
4)	Greene JR, et al. (1993) The GEF1 gene of Saccharomyces cerevisiae encodes an integral membrane protein; mutations in which have effects on respiration and iron-limited growth. Mol Gen Genet 241(5-6):542-53

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