ERG6/YML008C Summary Help

Standard Name ERG6 1
Systematic Name YML008C
Alias ISE1 , LIS1 , SED6 , VID1 2
Feature Type ORF, Verified
Description Delta(24)-sterol C-methyltransferase; converts zymosterol to fecosterol in the ergosterol biosynthetic pathway by methylating position C-24; localized to lipid particles, the plasma membrane-associated endoplasmic reticulum, and the mitochondrial outer membrane (1, 3, 4, 5, 6, 7 and see Summary Paragraph)
Name Description ERGosterol biosynthesis
Chromosomal Location
ChrXIII:252990 to 251839 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Genetic position: 2 cM
Gene Ontology Annotations All ERG6 GO evidence and references
  View Computational GO annotations for ERG6
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 9 genes
Classical genetics
reduction of function
Large-scale survey
605 total interaction(s) for 355 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 17
  • Affinity Capture-RNA: 3
  • Affinity Capture-Western: 5
  • Co-fractionation: 1
  • PCA: 64
  • Reconstituted Complex: 1
  • Two-hybrid: 5

Genetic Interactions
  • Dosage Rescue: 4
  • Negative Genetic: 380
  • Phenotypic Enhancement: 16
  • Phenotypic Suppression: 1
  • Positive Genetic: 62
  • Synthetic Growth Defect: 18
  • Synthetic Lethality: 12
  • Synthetic Rescue: 16

Expression Summary
Length (a.a.) 383
Molecular Weight (Da) 43,431
Isoelectric Point (pI) 5.6
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrXIII:252990 to 251839 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Genetic position: 2 cM
Last Update Coordinates: 1996-07-31 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..1152 252990..251839 1996-07-31 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004467

ERG6 encodes delta(24)-sterol C-methyltransferase, which converts zymosterol to fecosterol by methylating C-24 in the ergosterol biosynthetic pathway (1, 5, 3, 4). Cells lacking ERG6 are viable but unable to methylate sterols on C-24, and exhibit pleiotropic growth phenotypes (1). erg6 mutants show increased cation uptake (8), and are permeable and therefore sensitive to brefeldin A, a drug that disrupts secretion (9, 10). Enzymes similar to Erg6p have been identified in other organisms; a homolog from Candida albicans complements the S. cerevisiae erg6 null phenotype, and a homologous enzyme from Arabidopsis is active in yeast (11, 12).

Last updated: 2000-09-05 Contact SGD

References cited on this page View Complete Literature Guide for ERG6
1) Gaber RF, et al.  (1989) The yeast gene ERG6 is required for normal membrane function but is not essential for biosynthesis of the cell-cycle-sparking sterol. Mol Cell Biol 9(8):3447-56
2) Shieh HL, et al.  (2001) Biochemical analysis of fructose-1,6-bisphosphatase import into vacuole import and degradation vesicles reveals a role for UBC1 in vesicle biogenesis. J Biol Chem 276(13):10398-406
3) Lees ND, et al.  (1995) Cloning of the late genes in the ergosterol biosynthetic pathway of Saccharomyces cerevisiae--a review. Lipids 30(3):221-6
4) Parks LW, et al.  (1995) Biochemical and physiological effects of sterol alterations in yeast--a review. Lipids 30(3):227-30
5) Paltauf F, et al.  (1992) "Regulation and compartmentalization of lipid synthesis in yeast." Pp. 415-500 in The Molecular and Cellular Biology of the Yeast Saccharomyces: Gene Expression, edited by Jones EW, Pringle JR and Broach JR. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press
6) Pichler H, et al.  (2001) A subfraction of the yeast endoplasmic reticulum associates with the plasma membrane and has a high capacity to synthesize lipids. Eur J Biochem 268(8):2351-61
7) Zahedi RP, et al.  (2006) Proteomic analysis of the yeast mitochondrial outer membrane reveals accumulation of a subclass of preproteins. Mol Biol Cell 17(3):1436-50
8) Welihinda AA, et al.  (1994) Mutations in LIS1 (ERG6) gene confer increased sodium and lithium uptake in Saccharomyces cerevisiae. Biochim Biophys Acta 1193(1):107-17
9) Graham TR, et al.  (1993) Brefeldin A reversibly blocks early but not late protein transport steps in the yeast secretory pathway. EMBO J 12(3):869-77
10) Shah N and Klausner RD  (1993) Brefeldin A reversibly inhibits secretion in Saccharomyces cerevisiae. J Biol Chem 268(8):5345-8
11) Jensen-Pergakes KL, et al.  (1998) Sequencing, disruption, and characterization of the Candida albicans sterol methyltransferase (ERG6) gene: drug susceptibility studies in erg6 mutants. Antimicrob Agents Chemother 42(5):1160-7
12) Husselstein T, et al.  (1996) Transformation of Saccharomyces cerevisiae with a cDNA encoding a sterol C-methyltransferase from Arabidopsis thaliana results in the synthesis of 24-ethyl sterols. FEBS Lett 381(1-2):87-92