ERG5/YMR015C Summary Help

Standard Name ERG5 1
Systematic Name YMR015C
Alias CYP61 2
Feature Type ORF, Verified
Description C-22 sterol desaturase; a cytochrome P450 enzyme that catalyzes the formation of the C-22(23) double bond in the sterol side chain in ergosterol biosynthesis; may be a target of azole antifungal drugs (3, 4, 5, 6 and see Summary Paragraph)
Name Description ERGosterol biosynthesis 1
Chromosomal Location
ChrXIII:302485 to 300869 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gene Ontology Annotations All ERG5 GO evidence and references
  View Computational GO annotations for ERG5
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Regulators 16 genes
Classical genetics
reduction of function
Large-scale survey
266 total interaction(s) for 154 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 9
  • Affinity Capture-RNA: 4
  • Biochemical Activity: 1
  • PCA: 14

Genetic Interactions
  • Dosage Growth Defect: 1
  • Dosage Rescue: 1
  • Negative Genetic: 151
  • Phenotypic Enhancement: 2
  • Phenotypic Suppression: 1
  • Positive Genetic: 67
  • Synthetic Growth Defect: 11
  • Synthetic Lethality: 1
  • Synthetic Rescue: 3

Expression Summary
Length (a.a.) 538
Molecular Weight (Da) 61,334
Isoelectric Point (pI) 7.88
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrXIII:302485 to 300869 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..1617 302485..300869 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004617

ERG5 encodes C-22 sterol desaturase, a cytochrome P-450 enzyme that catalyzes the formation of the C-22(23) double bond in the sterol side chain in ergosterol biosynthesis (5, 1, 6, 3, 4). Cells lacking ERG5 are viable (1); an erg5 mutation increases ERG3 expression (7). Erg5p binds, and may be a target of, azole antifungal drugs (2). An ERG5 homolog has been identified in Candida glabrata (8).

Last updated: 2000-09-06 Contact SGD

References cited on this page View Complete Literature Guide for ERG5
1) Skaggs BA, et al.  (1996) Cloning and characterization of the Saccharomyces cerevisiae C-22 sterol desaturase gene, encoding a second cytochrome P-450 involved in ergosterol biosynthesis. Gene 169(1):105-9
2) Kelly SL, et al.  (1997) Characterization of Saccharomyces cerevisiae CYP61, sterol delta22-desaturase, and inhibition by azole antifungal agents. J Biol Chem 272(15):9986-8
3) Lees ND, et al.  (1995) Cloning of the late genes in the ergosterol biosynthetic pathway of Saccharomyces cerevisiae--a review. Lipids 30(3):221-6
4) Parks LW, et al.  (1995) Biochemical and physiological effects of sterol alterations in yeast--a review. Lipids 30(3):227-30
5) Kelly SL, et al.  (1995) Purification and reconstitution of activity of Saccharomyces cerevisiae P450 61, a sterol delta 22-desaturase. FEBS Lett 377(2):217-20
6) Paltauf F, et al.  (1992) "Regulation and compartmentalization of lipid synthesis in yeast." Pp. 415-500 in The Molecular and Cellular Biology of the Yeast Saccharomyces: Gene Expression, edited by Jones EW, Pringle JR and Broach JR. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press
7) Arthington-Skaggs BA, et al.  (1996) Positive and negative regulation of a sterol biosynthetic gene (ERG3) in the post-squalene portion of the yeast ergosterol pathway. FEBS Lett 392(2):161-5
8) Lamb DC, et al.  (1999) Purification, reconstitution, and inhibition of cytochrome P-450 sterol delta22-desaturase from the pathogenic fungus Candida glabrata. Antimicrob Agents Chemother 43(7):1725-8