DOA4/YDR069C Summary Help

Standard Name DOA4 1
Systematic Name YDR069C
Alias DOS1 2 , MUT4 3 , NPI2 4 , 5 , SSV7 6 , UBP4 7
Feature Type ORF, Verified
Description Ubiquitin hydrolase that deubiquitinates ILV cargo proteins; required for recycling ubiquitin from proteasome-bound ubiquitinated intermediates, acts at the late endosome/prevacuolar compartment to recover ubiquitin from ubiquitinated membrane proteins en route to the vacuole; DOA4 has a paralog, UBP5, that arose from the whole genome duplication (2, 8, 9, 10, 11)
Name Description Degradation Of Alpha 1
Chromosomal Location
ChrIV:587719 to 584939 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gene Ontology Annotations All DOA4 GO evidence and references
  View Computational GO annotations for DOA4
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 3 genes
Classical genetics
Large-scale survey
172 total interaction(s) for 123 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 14
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 6
  • Co-localization: 1
  • Co-purification: 2
  • PCA: 4
  • Reconstituted Complex: 1
  • Two-hybrid: 3

Genetic Interactions
  • Dosage Growth Defect: 1
  • Dosage Lethality: 2
  • Dosage Rescue: 5
  • Negative Genetic: 41
  • Phenotypic Enhancement: 12
  • Phenotypic Suppression: 32
  • Positive Genetic: 9
  • Synthetic Growth Defect: 4
  • Synthetic Lethality: 13
  • Synthetic Rescue: 21

Expression Summary
Length (a.a.) 926
Molecular Weight (Da) 105,191
Isoelectric Point (pI) 8.14
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrIV:587719 to 584939 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..2781 587719..584939 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002476
References cited on this page View Complete Literature Guide for DOA4
1) Papa FR and Hochstrasser M  (1993) The yeast DOA4 gene encodes a deubiquitinating enzyme related to a product of the human tre-2 oncogene. Nature 366(6453):313-9
2) Singer JD, et al.  (1996) Coordinating DNA replication to produce one copy of the genome requires genes that act in ubiquitin metabolism. Mol Cell Biol 16(4):1356-66
3) Grenson M and Acheroy B  (1982) Mutations affecting the activity and the regulation of the general amino-acid permease of Saccharomyces cerevisiae. Localisation of the cis-acting dominant pgr regulatory mutation in the structural gene of this permease. Mol Gen Genet 188(2):261-5
4) Jauniaux JC, et al.  (1987) Nitrogen catabolite regulation of proline permease in Saccharomyces cerevisiae. Cloning of the PUT4 gene and study of PUT4 RNA levels in wild-type and mutant strains. Eur J Biochem 164(3):601-6
5) Vandenbol M, et al.  (1987) Isolation of the NPR1 gene responsible for the reactivation of ammonia-sensitive amino-acid permeases in Saccharomyces cerevisiae. RNA analysis and gene dosage effects. Eur J Biochem 164(3):607-12
6) Xiao W, et al.  (1994) UBP5 encodes a putative yeast ubiquitin-specific protease that is related to the human Tre-2 oncogene product. Yeast 10(11):1497-502
7) van Nocker S, et al.  (1996) The multiubiquitin-chain-binding protein Mcb1 is a component of the 26S proteasome in Saccharomyces cerevisiae and plays a nonessential, substrate-specific role in protein turnover. Mol Cell Biol 16(11):6020-8
8) Swaminathan S, et al.  (1999) The Doa4 deubiquitinating enzyme is required for ubiquitin homeostasis in yeast. Mol Biol Cell 10(8):2583-94
9) Amerik AY, et al.  (2000) The Doa4 deubiquitinating enzyme is functionally linked to the vacuolar protein-sorting and endocytic pathways. Mol Biol Cell 11(10):3365-80
10) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
11) Richter CM, et al.  (2013) Doa4 function in ILV budding is restricted through its interaction with the Vps20 subunit of ESCRT-III. J Cell Sci 126(Pt 8):1881-90