CET1/YPL228W Summary Help

Standard Name CET1 1
Systematic Name YPL228W
Alias CES5 2
Feature Type ORF, Verified
Description RNA 5'-triphosphatase involved in mRNA 5' capping; subunit of the mRNA capping enzyme, which is a heterotetramer composed of a Cet1p homodimer and two molecules of the guanylyltransferase Ceg1p; Cet1p also has a role in regulation of RNA pol II pausing at promoter-proximal sites; interaction between Cet1p and Ceg1p is required for Ceg1p nuclear import; mammalian enzyme is a single bifunctional polypeptide; CET1 has a paralog, CTL1, that arose from the whole genome duplication (1, 3, 4, 5, 6, 7, 8, 9)
Name Description Capping Enzyme Triphosphatase 1
Chromosomal Location
ChrXVI:118382 to 120031 | ORF Map | GBrowse
Gene Ontology Annotations All CET1 GO evidence and references
  View Computational GO annotations for CET1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 2 genes
Classical genetics
Large-scale survey
reduction of function
117 total interaction(s) for 84 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 12
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 6
  • Co-crystal Structure: 2
  • Co-localization: 1
  • Co-purification: 1
  • Far Western: 1
  • PCA: 1
  • Protein-peptide: 1
  • Reconstituted Complex: 6
  • Two-hybrid: 2

Genetic Interactions
  • Dosage Rescue: 3
  • Negative Genetic: 59
  • Positive Genetic: 9
  • Synthetic Growth Defect: 4
  • Synthetic Lethality: 8

Expression Summary
Length (a.a.) 549
Molecular Weight (Da) 61,849
Isoelectric Point (pI) 5.36
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrXVI:118382 to 120031 | ORF Map | GBrowse
Last Update Coordinates: 1996-07-31 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..1650 118382..120031 1996-07-31 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000006149
References cited on this page View Complete Literature Guide for CET1
1) Tsukamoto T, et al.  (1997) Isolation and characterization of the yeast mRNA capping enzyme beta subunit gene encoding RNA 5'-triphosphatase, which is essential for cell viability. Biochem Biophys Res Commun 239(1):116-22
2) Ho CK, et al.  (1998) Genetic, physical, and functional interactions between the triphosphatase and guanylyltransferase components of the yeast mRNA capping apparatus. Mol Cell Biol 18(9):5189-98
3) Itoh N, et al.  (1984) Messenger RNA guanylyltransferase from Saccharomyces cerevisiae. I. Purification and subunit structure. J Biol Chem 259(22):13923-9
4) Shatkin AJ and Manley JL  (2000) The ends of the affair: capping and polyadenylation. Nat Struct Biol 7(10):838-42
5) Takagi T, et al.  (2002) Divergent subunit interactions among fungal mRNA 5'-capping machineries. Eukaryot Cell 1(3):448-57
6) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
7) Gu M, et al.  (2010) Structure of the Saccharomyces cerevisiae Cet1-Ceg1 mRNA Capping Apparatus. Structure 18(2):216-227
8) Lahudkar S, et al.  (2014) A Novel Role for Cet1p mRNA 5'-Triphosphatase in Promoter Proximal Accumulation of RNA Polymerase II in Saccharomyces cerevisiase. Genetics 196(1):161-76
9) Takizawa N, et al.  (2013) The Essential Role for the RNA Triphosphatase Cet1p in Nuclear Import of the mRNA Capping Enzyme Cet1p-Ceg1p Complex of Saccharomyces cerevisiae. PLoS One 8(10):e78000