CEG1/YGL130W Summary Help

Standard Name CEG1 1
Systematic Name YGL130W
Feature Type ORF, Verified
Description Guanylyltransferase involved in mRNA 5' capping; subunit of the mRNA capping enzyme, which is a heterotetramer composed of two molecules of Ceg1p and a homodimer of Cet1p, the mRNA 5?-triphosphatase subunit; nuclear import of Ceg1p requires interaction with Cet1p; mammalian capping enzyme is a single bifunctional polypeptide (1, 2, 3, 4, 5, 6, 7)
Name Description Capping Enzyme Guanylyltransferase 1
Chromosomal Location
ChrVII:266145 to 267524 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All CEG1 GO evidence and references
  View Computational GO annotations for CEG1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 4 genes
Resources
Classical genetics
conditional
null
Large-scale survey
null
reduction of function
unspecified
Resources
135 total interaction(s) for 88 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 35
  • Affinity Capture-RNA: 3
  • Affinity Capture-Western: 8
  • Co-crystal Structure: 1
  • Co-localization: 1
  • Co-purification: 1
  • Far Western: 1
  • Protein-peptide: 2
  • Reconstituted Complex: 4
  • Two-hybrid: 2

Genetic Interactions
  • Dosage Growth Defect: 2
  • Dosage Rescue: 13
  • Negative Genetic: 41
  • Positive Genetic: 5
  • Synthetic Growth Defect: 6
  • Synthetic Lethality: 8
  • Synthetic Rescue: 2

Resources
Expression Summary
histogram
Resources
Length (a.a.) 459
Molecular Weight (Da) 52,764
Isoelectric Point (pI) 5.63
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrVII:266145 to 267524 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1380 266145..267524 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003098
References cited on this page View Complete Literature Guide for CEG1
1) Shibagaki Y, et al.  (1992) mRNA capping enzyme. Isolation and characterization of the gene encoding mRNA guanylytransferase subunit from Saccharomyces cerevisiae. J Biol Chem 267(14):9521-8
2) Itoh N, et al.  (1984) Messenger RNA guanylyltransferase from Saccharomyces cerevisiae. I. Purification and subunit structure. J Biol Chem 259(22):13923-9
3) Shatkin AJ and Manley JL  (2000) The ends of the affair: capping and polyadenylation. Nat Struct Biol 7(10):838-42
4) Takagi T, et al.  (2002) Divergent subunit interactions among fungal mRNA 5'-capping machineries. Eukaryot Cell 1(3):448-57
5) Bougie I, et al.  (2004) Thermodynamics of ligand binding by the yeast mRNA-capping enzyme reveals different modes of binding. Biochem J 384(Pt 2):411-20
6) Gu M, et al.  (2010) Structure of the Saccharomyces cerevisiae Cet1-Ceg1 mRNA Capping Apparatus. Structure 18(2):216-227
7) Takizawa N, et al.  (2013) The Essential Role for the RNA Triphosphatase Cet1p in Nuclear Import of the mRNA Capping Enzyme Cet1p-Ceg1p Complex of Saccharomyces cerevisiae. PLoS One 8(10):e78000