BAR1/YIL015W Summary Help

Standard Name BAR1 1
Systematic Name YIL015W
Alias SST1 1 , 2
Feature Type ORF, Verified
Description Aspartyl protease; secreted into the periplasmic space of mating type a cell; helps cells find mating partners; cleaves and inactivates alpha factor allowing cells to recover from alpha-factor-induced cell cycle arrest (2, 3, 4 and see Summary Paragraph)
Name Description BARrier to the alpha factor response 1, 5
Chromosomal Location
ChrIX:322342 to 324105 | ORF Map | GBrowse
Gbrowse
Genetic position: -12 cM
Gene Ontology Annotations All BAR1 GO evidence and references
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 20 genes
Resources
Classical genetics
null
Large-scale survey
null
overexpression
Resources
20 total interaction(s) for 19 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 1
  • Affinity Capture-RNA: 3

Genetic Interactions
  • Dosage Rescue: 1
  • Negative Genetic: 6
  • Phenotypic Enhancement: 6
  • Synthetic Growth Defect: 2
  • Synthetic Rescue: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 587
Molecular Weight (Da) 63,729
Isoelectric Point (pI) 4.5
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrIX:322342 to 324105 | ORF Map | GBrowse
SGD ORF map
Genetic position: -12 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1994-12-10
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1764 322342..324105 2011-02-03 1994-12-10
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001277
SUMMARY PARAGRAPH for BAR1

Bar1p is a protease secreted into the periplasmic space of Mata cells that cleaves and inactivates alpha factor (2, 6). Mata cells that lack Bar1p are supersensitive to alpha factor-induced G1 arrest (2, 7). Transcription of BAR1 in alpha haploids and a/alpha diploids is repressed by the alpha2 protein and cofactors, and BAR1 transcription in Mata cells is stimulated by the presence of alpha factor (8, 9, 10). Bar1p is an aspartyl protease with similarity to pepsin, Pep4p, and Yap3p (11, 12, 6).

Last updated: 1999-12-31 Contact SGD

References cited on this page View Complete Literature Guide for BAR1
1) Sprague GF Jr and Herskowitz I  (1981) Control of yeast cell type by the mating type locus. I. Identification and control of expression of the a-specific gene BAR1. J Mol Biol 153(2):305-21
2) Chan RK and Otte CA  (1982) Physiological characterization of Saccharomyces cerevisiae mutants supersensitive to G1 arrest by a factor and alpha factor pheromones. Mol Cell Biol 2(1):21-9
3) Ciejek E and Thorner J  (1979) Recovery of S. cerevisiae a cells from G1 arrest by alpha factor pheromone requires endopeptidase action. Cell 18(3):623-35
4) Barkai N, et al.  (1998) Protease helps yeast find mating partners. Nature 396(6710):422-3
5) Hicks JB and Herskowitz I  (1976) Evidence for a new diffusible element of mating pheromones in yeast. Nature 260(5548):246-8
6) Ballensiefen W and Schmitt HD  (1997) Periplasmic Bar1 protease of Saccharomyces cerevisiae is active before reaching its extracellular destination. Eur J Biochem 247(1):142-7
7) Chan RK and Otte CA  (1982) Isolation and genetic analysis of Saccharomyces cerevisiae mutants supersensitive to G1 arrest by a factor and alpha factor pheromones. Mol Cell Biol 2(1):11-20
8) Kronstad JW, et al.  (1987) A yeast operator overlaps an upstream activation site. Cell 50(3):369-77
9) Manney TR  (1983) Expression of the BAR1 gene in Saccharomyces cerevisiae: induction by the alpha mating pheromone of an activity associated with a secreted protein. J Bacteriol 155(1):291-301
10) Hagen DC, et al.  (1991) Pheromone response elements are necessary and sufficient for basal and pheromone-induced transcription of the FUS1 gene of Saccharomyces cerevisiae. Mol Cell Biol 11(6):2952-61
11) MacKay VL, et al.  (1988) The Saccharomyces cerevisiae BAR1 gene encodes an exported protein with homology to pepsin. Proc Natl Acad Sci U S A 85(1):55-9
12) Egel-Mitani M, et al.  (1990) A novel aspartyl protease allowing KEX2-independent MF alpha propheromone processing in yeast. Yeast 6(2):127-37