ADE1/YAR015W Summary Help

Standard Name ADE1 1
Systematic Name YAR015W
Feature Type ORF, Verified
Description N-succinyl-5-aminoimidazole-4-carboxamide ribotide synthetase; required for 'de novo' purine nucleotide biosynthesis; red pigment accumulates in mutant cells deprived of adenine; protein abundance increases in response to DNA replication stress (2, 3, 4)
Name Description ADEnine requiring 1
Chromosomal Location
ChrI:169375 to 170295 | ORF Map | GBrowse
Gbrowse
Genetic position: 4 cM
Gene Ontology Annotations All ADE1 GO evidence and references
  View Computational GO annotations for ADE1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
High-throughput
Regulators 15 genes
Resources
Pathways
Classical genetics
null
Large-scale survey
null
unspecified
Resources
43 total interaction(s) for 29 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 8
  • Affinity Capture-RNA: 1
  • Biochemical Activity: 1
  • PCA: 2
  • Reconstituted Complex: 1
  • Two-hybrid: 1

Genetic Interactions
  • Negative Genetic: 23
  • Positive Genetic: 3
  • Synthetic Growth Defect: 3

Resources
Expression Summary
histogram
Resources
Length (a.a.) 306
Molecular Weight (Da) 34,603
Isoelectric Point (pI) 5.8
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrI:169375 to 170295 | ORF Map | GBrowse
SGD ORF map
Genetic position: 4 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..921 169375..170295 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000070
References cited on this page View Complete Literature Guide for ADE1
1) Dorfman BZ  (1969) The isolation of adenylosuccinate synthetase mutants in yeast by selection for constitutive behavior in pigmented strains. Genetics 61(2):377-89
2) Myasnikov AN, et al.  (1991) The Saccharomyces cerevisiae ADE1 gene: structure, overexpression and possible regulation by general amino acid control. Gene 109(1):143-7
3) Jones EW and Fink GR  (1982) "Regulation of amino acid and nucleotide biosynthesis in yeast." Pp.181-299 in The Molecular Biology of the Yeast Saccharomyces: Metabolism and Gene Expression, edited by Strathern JN, Jones EW and Broach JR. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press
4) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76