ZDS1/YMR273C Summary

ZDS1 BASIC INFORMATION

Standard Name	ZDS1 1, 2
Systematic Name	YMR273C
Alias	CES1 3 , CKM1 , NRC1 , OSS1 4
Feature Type	ORF, Verified
Description	Protein that interacts with silencing proteins at the telomere, involved in transcriptional silencing; has a role in localization of PKA subunit Bcy1p; implicated in mRNA nuclear export; involved in mitotic exit through regulation of Cdc14p (2, 5, 6, 7, 8)
Name Description	Zillion Different Screens 1, 2

GO Annotations	All ZDS1 GO evidence and references
	View Computational GO annotations for ZDS1
Molecular Function
Manually curated	protein phosphatase type 2A regulator activity (IGI, IMP)
Biological Process
Manually curated	cell aging (IMP) chromatin silencing (IMP) establishment of cell polarity (IGI, IMP) mRNA export from nucleus (IGI, IMP, IPI) regulation of protein localization (IMP)
Cellular Component
Manually curated	cellular bud tip (IDA) cytoplasm (IPI) incipient cellular bud site (IDA) nuclear pore (IPI)

Regulatory Role
Regulatory modules	predicted: stressResponse ( 529 ) predicted: cellcycle ( 323 ) predicted: stressResponse ( 529 ) predicted: cellcycle ( 323 )

Mutant Phenotype	All ZDS1 Phenotype details and references
Classical genetics
null	bud morphology: abnormal chromosome/plasmid maintenance: increased Slt2p accumulation: increased Cdc14p distribution: delayed GFP-HA-Bcy1p distribution: abnormal Sir3p modification: decreased GFP-HA-Bcy1p modification: increased Slt2p modification: increased replicative lifespan: increased resistance to calcium dichloride: decreased silencing: decreased silencing: increased viable
overexpression	actin cytoskeleton morphology: abnormal bud morphology: abnormal cell shape: abnormal cell size: increased chromosome/plasmid maintenance: increased heat sensitivity: increased GFP-HA-Bcy1p distribution: abnormal silencing: increased silencing: decreased
Large-scale survey
null	filamentous growth: increased resistance to rapamycin: decreased resistance to wortmannin: normal resistance to calcium dichloride: decreased viable

Interactions	ZDS1 All interactions details and references
	118 total interaction(s) for 79 unique genes/features.
Physical Interactions	Affinity Capture-MS: 31 Affinity Capture-RNA: 2 Affinity Capture-Western: 3 Reconstituted Complex: 2 Two-hybrid: 12
Genetic Interactions	Dosage Lethality: 3 Dosage Rescue: 21 Phenotypic Enhancement: 15 Phenotypic Suppression: 17 Synthetic Growth Defect: 4 Synthetic Lethality: 4 Synthetic Rescue: 4

Sequence Information

ChrXIII:813979 to 811232 | |

Note: this feature is encoded on the Crick strand.

Last Update

Coordinates: 2004-02-27 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..2748	813979..811232	2004-02-27	1996-07-31

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| UniProtKB

Primary SGDID	S000004886

ZDS1 RESOURCES

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SGD ORF map	GBrowse

Click on histogram for expression summary
Expression Summary

ADDITIONAL INFORMATION for ZDS1

NOMENCLATURE CONFLICT NOTE

Name	Relevance	Description
OSS1	Nomenclature conflict	OSS1 has been used in the literature to refer to both ZDS1/YMR273C, which encodes a protein involved in transcriptional silencing and polarity establishment, and OSS1, a genetically defined locus on the mitochondrial chromosome that confers ossamycin resistance when mutated.

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for ZDS1]

1)	Yu Y, et al. (1996) Mutations in the homologous ZDS1 and ZDS2 genes affect cell cycle progression. Mol Cell Biol 16(10):5254-63
2)	Bi E and Pringle JR (1996) ZDS1 and ZDS2, genes whose products may regulate Cdc42p in Saccharomyces cerevisiae. Mol Cell Biol 16(10):5264-75
3)	Schwer B and Shuman S (1996) Multicopy suppressors of temperature-sensitive mutations of yeast mRNA capping enzyme. Gene Expr 5(6):331-44
4)	Ma XJ, et al. (1996) A search for proteins that interact genetically with histone H3 and H4 amino termini uncovers novel regulators of the Swe1 kinase in Saccharomyces cerevisiae. Genes Dev 10(11):1327-40
5)	Roy N and Runge KW (1999) The ZDS1 and ZDS2 proteins require the Sir3p component of yeast silent chromatin to enhance the stability of short linear centromeric plasmids. Chromosoma 108(3):146-61
6)	Griffioen G, et al. (2001) Nucleocytoplasmic distribution of budding yeast protein kinase A regulatory subunit Bcy1 requires Zds1 and is regulated by Yak1-dependent phosphorylation of its targeting domain. Mol Cell Biol 21(2):511-23
7)	Estruch F, et al. (2005) Physical and genetic interactions link the yeast protein Zds1p with mRNA nuclear export. J Biol Chem 280(10):9691-7
8)	Queralt E and Uhlmann F (2008) Separase cooperates with Zds1 and Zds2 to activate Cdc14 phosphatase in early anaphase. J Cell Biol 182(5):873-83

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